HPLC测复方野菊花含片中黄芩苷和蒙花苷含量  

Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC

作　　者：陈红英[1,2] 倪健[1] 张辉[2] 

机构地区：[1]北京中医药大学,北京100190 [2]华润三九医药股份有限公司,深圳518110

出　　处：《中国实验方剂学杂志》2013年第10期122-124,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立复方野菊花含片中黄芩苷和蒙花苷含量测定的方法。方法:采用HPLC,Kromasil-C18(4.6 mm×250 mm,5μm)色谱柱,流动相乙腈-0.1%磷酸(25∶75),检测波长334 nm。结果:黄芩苷和蒙花苷进样量分别在0.460～2.760μg(r=0.999 5),0.020～0.400μg(r=0.999 9)线性关系良好;黄芩苷和蒙花苷平均回收率分别为101.77%(RSD0.98%),98.36%(RSD 1.68%)。结论:该方法简便易行,重复性好,可用于复方野菊花含片中黄芩苷和蒙花苷含量测定。Objective： To establish a RP-HPLC method for simultaneous determination of baicalin and buddleoside in Compound Yejuhua Buccal Tablets. Method： HPLC was adopted to determine the content of baicalin and buddleoside. The Kromasil-C18 Column （4.6 mm×250 mm, 5 μm） was used with the mobile phase consisting of acetonitrile-0.1% aqueous phosphoric acid （25：75）; the detection wavelength was set at 334 nm. Result： There was good linearity between the peak area and concentration within the range of 0.460-2.760 μg （r=0.999 9） for baicalin and 0.020-0.400 μg （r=0.999 9） for buddleoside respectively. The average recoveries of baicalin and buddleoside were 101.77% （RSD 0.98%） and 98.36% （RSD 1.68%） respectively （n=6）. Conclusion： The method is simple, accurate and specific for quality control of Compound Yejuhua Buccal Tablets.

关 键 词：复方野菊花含片 黄芩苷 蒙花苷 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]