HPLC鉴别哮喘宁片中五味子与南五味子  

Identification of Fructus Schisandra chinensis and Fructus Schisandra Sphenanthera in Xiaochuanning Tablets by HPLC

作　　者：张汶婕 杨惠 万庆 陈叶平 

机构地区：[1]铜陵市食品药品检验所,安徽铜陵244000

出　　处：《中国实验方剂学杂志》2013年第10期153-155,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:鉴别哮喘宁片中五味子与南五味子。方法:以五味子酯甲、五味子醇甲、五味子甲素、五味子乙素为对照,采用SunFireTMC18柱(4.6 mm×150 mm,5μm),乙腈-四氢呋喃-水梯度洗脱,检测波长254 nm,流速1.0 mL.min-1,用中国药典委员会颁布的中药色谱指纹图谱相似度评价系统软件(2004A)进行相似度的比较。结果:五味子药材及其成药制剂、南五味子药材及其成药制剂指纹图谱相似度均<0.9,但五味子、南五味子指纹图谱相似度<0.2。结论:采用色谱技术能够鉴别哮喘宁片中五味子与南五味子。Objective： To identify Fructus Schisandra chinensis and Fructus Schisandra Sphenanthera in Xiaochuanning Tablets. Method： Schisantherin A, schisandrin, deoxyschizandrin, schizandrin B served as the reference substances. The assay was conducted on SunFireTM C18 column （4.6 mm×150 mm, 5 μm） with acetonitrile-tetrahydrofuran-water as mobile phase. The flow rate was 1.0 mLmin-1 and the detection wavelength was set at 254 nm. Similarity evaluation system for chromatographic fingerprint of TCM （2004 A） was used in data analysis. Result： Fructus Schisandra Chinensis from habitats were of strong similarity with those from Xiaochuanning Tablets, but the similarities with Fructus Schisandra Sphenanthera from habitats or tablets were less than 0.20. Conclusion： HPLC can be used for identification of Fructus Schisandra Chinensis and Fructus Schisandra Sphenanthera in Xiaochuanning Tablets.

关 键 词：五味子 南五味子 哮喘宁片 液相色谱法 

分 类 号：R284.1[医药卫生—中药学]