基于尿液生成排泄探讨缩泉丸配伍机制  

Study on Compatibility of Suoquan Pill based on Excretion of Urine

作　　者：邱琼华[1] 吴君[2] 赖焕玲[1] 黄萍[1] 操红缨[1] 吴清和[1] 

机构地区：[1]广州中医药大学,广州510006 [2]山东中医药高等专科学校,山东烟台264100

出　　处：《中国实验方剂学杂志》2013年第10期207-210,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2010B030700025)

摘　　要：目的:探讨缩泉丸配伍的机制,确定各味药物在方中的作用。方法:对缩泉丸进行全拆方研究,正常对照组、模型对照组、缩泉丸组(3.9 g.kg-1.d-1)、益智组、乌药组、山药组(均为1.3 g.kg-1.d-1),益智+乌药组、益智+山药组、乌药+山药组(均为2.6 g.kg-1.d-1)。除正常对照组外各组用D-半乳糖诱导亚急性衰老模型,造模给药后各组用尿动力仪检测膀胱功能,用ELISA法测定与尿液生成相关的水通道蛋白(AQP2),血清中神经内分泌激素醛固酮(ALD)、抗利尿激素(ADH)的含量。结果:与正常组比较,模型组AQP2,ADH,ALD含量显著降低(P<0.01),尿动力检测中膀胱漏尿点压(BLPP)、腹压漏尿点压(ALPP)、最大膀胱排尿压(MVP)显著升高(P<0.01),顺应性(BC)显著下降(P<0.01)。缩泉丸全方对所测指标都有显著影响(与模型组比较P<0.01),且与各拆方组比较作用最强。单味药比较益智作用最强,乌药对尿液生成方面作用显著,与益智配伍后增效;山药能显著改善衰老膀胱功能,与乌药配伍后药效显著增强,对所测指标均有显著作用(与模型组比较P<0.01～0.05)。结论:本研究结果与缩泉丸益智仁为君,乌药为臣,山药为佐的传统配伍诠释相符。Objective： To study the compatibility of the Suoquan pill based on excretion of urine. Method： The combinations including Alpiniae Oxyphyllae Fructus, Linderae Radix, Dioscoreae Rhizoma,Alpiniae Oxyphyllae Fructus＋Linderae Radix,Alpiniae Oxyphyllae Fructus＋Dioscoreae Rhizoma,Linderae Radix＋Dioscoreae Rhizoma,Linderae Radix＋Dioscoreae Rhizoma and Suoquan pill.Treated respectively with the combination of herbs and D-gal,seted up normal group at the same time.To assess the bladder function by urodynamics and measure the aquaporin 2（AQP2）,aldosterone （ALD）,antidiuretic hormone （ADH） associated with the urine formation by ELISA technology. Result： Compared with the normal group, model group AQP2,ADH,ALD content significantly reduced （P〈0.01）,Bladder Leak Point Pressures（BLPP）,abdomen leak point pressure（ALPP）,maximum voiding pressure（MVP）significantly reduced （P〈0.01）,also,and the bladder compliance（BC）elevated （P〈0.01）.Among separated recipes groups,Suoquan pill was better than other groups.Alpiniae Oxyphyllae Fructus has strongest efficacy of single herb;Linderae Radix was significant effects in the urine formation,combination of Alpiniae Oxyphyllae Fructus has better efficacy. Dioscoreae Rhizoma plays an important part in ameliorating the senescent bladder function,combination of Linderae Radix has better efficacy in all we check. Conclusion： The result is conformed to the theory of traditional Chinese medicine.

关 键 词：缩泉丸 配伍机制 尿液生成 膀胱功能 

分 类 号：R285.5[医药卫生—中药学]