姬松茸多糖对大鼠胸腺细胞凋亡和线粒体膜电位的影响  

Effect of Agaricus blazei Polysaccharide on Thymocyte Apoptosis and Mitochondrial Membrane Potential in Rats

作　　者：黄清松[1] 李红枝[1] 陈爱葵[2] 

机构地区：[1]广东药学院基础学院,广州510006 [2]广东第二师范学院生物系,广州510303

出　　处：《中国实验方剂学杂志》2013年第10期232-235,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察姬松茸多糖对地塞米松(dexamethasone,DEX)诱发大鼠胸腺细胞凋亡和线粒体膜电位的影响。方法:Wistar大鼠随机分为4组:正常组,模型组,姬松茸多糖低、高剂量组,每组10只。正常组和模型组灌胃(ig)生理盐水,姬松茸多糖低、高剂量组ig姬松茸多糖溶液(60,120 mg.kg-1),1次/d,连续7 d。模型组和姬松茸多糖低、高剂量于第7日腹腔注射DEX(0.02 g.kg-1)。3 h后处死各组动物,迅速无菌取胸腺,采用显微观察、流式细胞技术观察胸腺细胞形态学改变,胸腺细胞凋亡、脱氧核糖核酸(DNA)断裂百分率及线粒体膜电位变化,胸腺组织超氧化物歧化酶(SOD)、丙二醛(MDA)的变化。结果:与模型组比较,姬松茸多糖组大鼠胸腺细胞线粒体嵴肿胀明显减轻;姬松茸多糖组大鼠胸腺细胞凋亡率[低、高剂量(5.96±1.14)%,(5.07±1.02)%],DNA断裂率[低、高剂量(3.67±0.49)%,(3.24±0.45)%]及胸腺组织MDA含量[低、高剂量(1.87±0.25),(1.72±0.23)μg.mg-1]均明显低于模型组[(7.14±1.62)%,(4.25±0.86)%,(2.16±0.38)μg.mg-1];姬松茸多糖组大鼠胸腺细胞线粒体膜电位(荧光指数:低剂量38.57±6.29,高剂量41.36±7.18)及胸腺组织SOD活性量[低、高剂量(25.78±3.94),(27.36±4.21)U.mg-1]明显高于模型组(32.76±5.48)荧光指数,(21.45±3.52)μg.mg-1。结论:姬松茸多糖可阻断胸腺细胞DNA断裂和线粒体膜电位的下降,有效抑制DEX诱导的胸腺细胞凋亡,其作用机制与姬松茸多糖提高胸腺组织抗氧化功能有关。Objective： To investigate effects of Agaricus blazei polysaccharide （ABP） on rat thymocyte apoptosis and mitochondrial membrane potential which were induced by dexamethasone （DEX）. Method： Forty Wistar rats were divided into four groups randomly with 10 in each group. The four groups were as follows： normal group, model group, low dose ABP group and high dose ABP group.Normal group and model group were prepared by normal saline solution; low dose ABP group and high dose ABP group were prepared by ABP solution（60,120 mgkg-1）, once a day for seven days continuously. Model group and low dose ABP group were injected through belly by DEX（0.02 gkg-1）. All groups were killed three hours later and rat thymocyte was taken out clenly. Mcroscopic observation and flow cytometry were used to observe morph ological changes and apoptosis of rat thymocyte,percentage of deoxyribonucleic acid（DNA） strand breakage,mitochondrial membrane potential changes and contents of superoxide dismutase （SOD） in rat thymocyte and malomialdehvde （MDA）. Result： Mitochondria cristal dilatation of thyocyte obviously released in ABP group compared with model group. Rate of rat thymocyte apoptosis in ABP group [low dose（5.96±1.14）%,high dose（5.07±1.02）%], DNA breakage rate[low dose（3.67±0.49）%,high dose（3.24±0.45）%]and MDA content in rat thymocyte [low dose（1.87±0.25）μgmg-1,high dose（1.72±0.23）μgmg-1] were obviously lower than model group[（7.14±1.62）%,（4.25±0.86）%,（2.16±0.38）μgmg-1]; mitochondrial membrane potential in rat thymocyte （fluorescence index：low dose 38.57±6.29,high dose 41.36±7.18）and SOD conten in rat thymocyte [low dose（25.78±3.94）μgmg-1,high dose（27.36±4.21）μgmg-1]; mitochondrial membrane potential in rat thymocyte （fluorescence index：low dose 38.57±6.29,high dose 41.36±7.18）and SOD conten in rat thymocytemg-1,high dose（27.36±4.21）μgmg-1]were significantly higher than model group（32.76±5.48） fluorescence index,（2

关 键 词：姬松茸多糖 胸腺细胞 凋亡 线粒体膜电位 

分 类 号：R285.5[医药卫生—中药学]