醒脑再造胶囊对血管性痴呆模型大鼠的保护作用  

Protective Effects of Xingnao Zaizao Capsules on Vascular Dementia in Rats

作　　者：任莹[1] 刘静[2] 景小龙[2] 申秀萍[2] 田义红[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津药物研究院,天津300301

出　　处：《中国实验方剂学杂志》2013年第10期242-245,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察醒脑再造胶囊对血管性痴呆大鼠的保护作用。方法:采用永久性结扎双侧颈总动脉结合眼眶放血法制备大鼠VD模型。术后第3天,进行Y迷宫检测,根据结果将手术动物随机分为模型组、甲磺酸双氢麦角碱(5 mg.kg-1.d-1)、醒脑再造胶囊高、中、低(0.4,0.2,0.1 g.kg-1.d-1)剂量组,另有只分离血管不结扎的设为假手术组,每组10只。第4天开始ig给药,5 mL.kg-1,1次/d,给药3周。通过Y迷宫检测其学习记忆能力,HPLC法检测脑组织内的递质含量,激光多普勒血流量仪检测脑血流速度。结果:Y迷宫检测模型组的正确次数显著低于假手术组(P<0.01),阳性药甲磺酸双氢麦角碱组和醒脑再造胶囊高、中剂量组大鼠的正确次数较模型组明显提高(P<0.01或P<0.05);模型组平均脑血流速度223.5mm.s-1,醒脑再造胶囊3个剂量组较模型显著提高;与正常组比较模型组脑组织去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)3种递质含量降低(P<0.01),阳性药组和醒脑再造胶囊3个剂量组均较模型组显著提高(P<0.01或P<0.05)。结论:醒脑再造胶囊能显著改善血管性痴呆大鼠学习记忆能力,提高脑组织内单胺类递质含量和脑血流量。Objective： To investigate the protective effects of Xingnao Zaizao capsules on vascular dementia in rats. Method： The rats were divided into sham-operation group,model group,Co-dergocrine Mesylate group（5 mgkg-1）and three Xingnao Zaizao capsules groups at high,median and low doses（0.4,0.2,0.1 gkg-1d-1）.VD rat models were reproduced by bilateral carotid artery occlusion.The learning and memory was assayed by Y maze.The cerebral blood flow was measured with the help of Laser Doppler flowmeter. At the end of the experiment,the rats were sacrificed and samples of brain tissues were used to determine the monoamine neurotransmitters by HPLC-FLD. Result： The time of correct response in the model group was significantly less than sham-operation group whose （P〈0.01 or P〈0.05）,and administered with Co-dergocrine Mesylate and Xingnao Zaizao capsules increased the correct response（P〈0.01）.Also, the cerebral blood flow were increased （P〈0.05）,NE,DA,5-HT in brain tissueswere increased remarkably compared with model group （P〈0.01 or P〈0.05）. Conclusion： Xingnao Zaizao capsules ameliorate the ability of learning and memory in VD rats, increase thecerebral blood flow,monoamineneurotransmittersin brain tissues.

关 键 词：醒脑再造胶囊 血管性痴呆 单胺类神经递质 脑血流量 

分 类 号：R285.5[医药卫生—中药学]