肾阳虚外感小鼠模型的建立及评价  

Establishment and Evaluation of Mice Model with Kidney Yang Deficiency Infected by Influenza Virus

作　　者：尹翠翠[1] 杨勇[1] 李静[1] 李荣荣[1] 容蓉[1] 孟红[2] 

机构地区：[1]山东中医药大学,济南250355 [2]山东省医学科学院基础医学研究所,济南250062

出　　处：《中国实验方剂学杂志》2013年第10期253-256,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81273626);山东省中医药管理局项目(2011-027);山东省高等学校科技计划项目(J10LF20)

摘　　要：目的:建立肾阳虚外感复合病证小鼠模型。方法:腹腔注射苯甲酸雌二醇注射液(0.008 mg.g-1),复制小鼠肾阳虚模型。在此基础上,采用鼠肺病毒株FM1滴鼻建立肾阳虚小鼠外感模型,观察小鼠体征表现,死亡率及每天体重、游泳时间、肛温变化;测定小鼠血清肿瘤坏死因子-α(TNF-α),干扰素-γ(IFN-γ)含量;并观察肺组织病理变化;测肾、肺、脾、肾上腺及胸腺的脏器指数。结果:模型组小鼠游泳时间、肛温、趾温、自主活动均明显下降(P<0.01);肺组织出现外感病毒性肺炎的特征;与正常对照组比较,模型组小鼠各脏器指数均降低,其中胸腺具有显著性差异(P<0.01);IFN-γ含量明显提高(P<0.05);TNF-α含量有降低趋势。结论:采用雌二醇腹腔注射结合鼻腔滴注流感病毒FM1,病证结合建立的肾阳虚外感病证小鼠模型,在一定程度上反映了中医肾阳虚外感的特点,为研究肾阳虚外感的发病机制及药物干预提供了较理想的实验模型。Objective： To establish a mouse model with kidney Yang deficiency infected by influenza virus. Method： Kidney Yang deficiency mice were established by intraperitoneal injection of estradiol benzoate （0.008 mgg-1） as previously described. The physical signs in mice were observed, and the swimming time of mice, rectal temperature, toe temperature, autonomic mobility were examined.On the basis of above model, Mice were inoculated with mouse lung influenza virus （FM1） by the nasal cavity to establish the mice model with kidney Yang deficiency infected by influenza virus. Then physical signs, mortality, body weight and temperature changes were observed daily.The concentrations of tumor necrosis factor-α （TNF-α） and interferon-γ （IFN-γ） in mouse serum were measured, and the organ indexes of lung, spleen,kidney, adrenal and thymus were detected. Pathological changes of lung were observed. Result： The swimming time, rectal temperature, toe temperature, and autonomic activities of mice model were significantly decreased compared with normal group （P〈0.01）; Viral pneumonia was detected in lung tissue. Compared with the normal control, the thymus index of model mice was remarkably reduced （P〈0.01）. The level of IFN-γ was obviously increased （P〈0.05） and TNF-α level was decreased. Conclusion： To some extent, the symptoms of kidney-yang deficiency with influenza disease could embodied in this mouse model, which was successfully established by combining estradiol intraperitoneal injection with nasal cavity by influenza virus FM1 and this provided a more ideal experimental model for pathogenesis and drug intervention.

关 键 词：流感病毒 苯甲酸雌二醇 肾阳虚外感模型 评价 

分 类 号：R285.5[医药卫生—中药学]