高效液相色谱法测定鱼肉中的碱性橙Ⅱ和碱性嫩黄O两种工业染料  

Determination of chrysoidin Ⅱ and auramine O in fish via high performance liquid chromatography

作　　者：黄雅佩[1] 吴官非 夏焜[1] 王焦清[1] 曹旭妮[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室食品系,上海200237

出　　处：《化学试剂》2013年第5期435-438,共4页Chemical Reagents

基　　金：上海市科委资助项目(12142201300;1214220-0803;11142201200;11142200703;10142200803;11nm05-05200);上海市教委资助重点项目(12ZZ048)

摘　　要：碱性橙Ⅱ和碱性嫩黄O均为工业用着色剂,不能作为食品添加用色素。为此建立了鱼肉样品中同时检查碱性橙Ⅱ和碱性嫩黄O的高效液相色谱定量分析方法,以甲醇-0.03%磷酸水溶液(pH 2.0)为流动相,Hydrosphere-C18色谱柱分离,二极管阵列检测器检测,检测波长为440 nm,可以实现鱼肉样品中添加的碱性橙Ⅱ和碱性嫩黄O的含量分析。该方法碱性橙Ⅱ和碱性嫩黄O的线性范围均为0.1～10μg/mL,最低检出浓度均为0.04μg/mL,方法的回收率为95%～116%,相对标准偏差(RSD)为2.0%～3.5%。此外,还进一步考察了前处理方法中采用磷酸-甲醇直接研磨鱼肉并抽提被分析物,抽提液适当蒸发浓缩,可以实现鱼肉样品中添加0.25μg/g的碱性橙Ⅱ和碱性嫩黄O的分析检测,以及自制浸泡黄鱼样品中的碱性橙Ⅱ和碱性嫩黄O的分析检测。A method for the determination of ehrysoidin Ⅱ and auramine O in fish was established via high performance liquid chromatography （HPLC）. The separation was per- formed in a YMC hydrosphere-C18 （250 mm ×4.6 mm i. d. 5 μm） column, methanol and 0.03% H3PO4 water solution were used as mobile phase. A photo-diode array detector was used to detect them at 440 nm. The linear range was O. 1 × 1.0 μg=/mL, and the detection limit was 0. 04 μg./mL. The re- covery ranged from 95% to 116% and RSD was 2. 0% -3. 5%. chrysoidin Ⅱ and auramine O were successfully detected at the level of 0.25 μg/g in fish after extracted by H3PO4-meth- anol solution and then concentrated by evaporation.

关 键 词：碱性橙Ⅱ 碱性嫩黄O 高效液相色谱 鱼 

分 类 号：O657.7[理学—分析化学]