过量表达水杨酸羟基酶NahG降低烟草对青枯菌的抗性  

Overexpression of salicylate hydroxylase NahG declines resistance to Ralstonia solanacearum infection in tobacco

作　　者：党峰峰[1] 林金辉[1] 陈成聪[1] 陈永萍[2] 官德义[3] 何水林[1,3] 

机构地区：[1]福建农林大学生命科学学院,福州35002 [2]福建农林大学园艺学院,福州35002 [3]福建农林大学作物遗传改良和综合利用教育部重点实验室,福州35002

出　　处：《广西植物》2013年第2期177-180,198,共5页Guihaia

基　　金：国家自然科学基金(30971718);国家高等教育博士研究基因重大专项(20093535110004);转基因重大专项(2009ZX08001-015B);福建省自然科学基金(2008J049)

摘　　要：研究构建了pMDC32-NahG植物表达载体,并获得了其超表达的转基因烟草T1代株系。结果表明:这些株系与其野生型植株没有明显表型差异,但却表现出较野生型植株更敏感的青枯菌侵染抗性。同时,研究还发现NahG的超表达还显著提高了茉莉酸(JA)-依赖的基因NtPR1-b的表达,降低了(SA)-依赖的相关基因NtPR3和NtPRQ的表达。这表明SA累积的缺陷降低了烟草对青枯菌侵染的抗性。Studies showed that the plant expression vector of pMDC32-NahG was construed in tobacco K326,and ob- tained NahG-OE T1 lines. Comparing to wild type K326, NahG-OE lines did not exhibited any phenotypic differ- ence, but exhibited higher susceptibility to Ralstonia solanacearum inoculation. Meanwhile, it was found to be coupled with up-regulated expression of JA-depended NtPRI-b, and down-regulated expression of SA-depended NtPR3 and NtPRQ. Results suggested that SA-degrading NahG tobacco plants declined resistance to R. solanacearum infection.

关 键 词：水杨酸 NAHG 青枯菌 

分 类 号：Q943.2[生物学—植物学]