甘蔗细茎野生种(S.spontaneum)F1代花粉低温贮存研究  

Pollen storage of F 1 S. spontaneum at low temperature

作　　者：蔡青[1] 范源洪[1] 夏红明[1] 王丽萍[1] 马丽[1] 

机构地区：[1]云南省农科院甘蔗研究所,云南开远661600

出　　处：《甘蔗（福建）》2000年第3期1-7,共7页Sugarcane

基　　金：国家攻关专题! (96 - 0 0 2 - 0 2 - 15)

摘　　要：5个甘蔗细茎野生种 (S.spontaneum) F1代花粉经 2种不同干燥方式和 2种不同贮存温度处理 ,授予花粉发育率低且自交无结实的栽培品种母本花穗进行杂交 ,其中 9个花穗育出实生苗 37株 ,经过氧化物酶同工酶鉴定 ,95.2 %的实生苗为真实杂交后代。结果表明 :本试验条件下 ,低温贮存花粉可保持活性时间最长达 12 8天 ,但以 35- 50天的贮存花粉活性较好 ;在 4种贮存方法中 ,硅胶干燥的花粉在低温冰箱 (- 2 8至 - 30℃ )中贮存和经抽湿干燥的花粉在液氮 (- 196℃ )Pollen of 5 F 1 clones of S. spontaneum was treated with 2 methods and stored in 2 levels of temperature, then pollinated to commercial varieties of pollen lacking and self sterility. Identified with peroxidase isozyme, 95.2% of 37 seedings produced from the 9 crosses were true hybrid progeny. The results showed that the viability of pollen stored at low temperature might last 128 days but the optimal was 35-50 days. Two better methods were low temperature refrigerator (-28 to -30 ℃) storage after drying by silica gel and liquid nitrogen storage (-196℃) after drying by dehumidifier.

关 键 词：甘蔗 细茎野生种 花粉低温贮存 种质资源 

分 类 号：S566.102.4[农业科学—作物学]