黄花忍冬花和果实中金丝桃苷含量的测定  

Determination of the Contents of Hyperoside in Flowers and Fruits of Lonicera chrysantha

作　　者：董长颖[1] 于加平[1] 于海欢[1] 

机构地区：[1]吉林农业科技学院,吉林吉林132109

出　　处：《贵州农业科学》2013年第4期34-36,共3页Guizhou Agricultural Sciences

基　　金：吉林省教育厅"十二五"科学技术研究项目"黄花忍冬主要活性成分的提取及活性研究"[吉教科合字(2011)第272号]

摘　　要：为给黄花忍冬的药用开发及综合利用提供依据,采用索氏提取法,建立了黄花忍冬花和果实中金丝桃苷的提取方法,并运用RP-HPLC法测定了提取液中金丝桃苷的含量。结果表明:金丝桃苷在0.300～1.200μg内与峰面积呈良好的线性关系,r=0.999 6,平均加标回收率为99.42%,RSD为0.313%。该方法简便、可靠、重复性好。In order to provide the basis for development and comprehensive utilization of L. chrysantha, the author established the extraction method of hyperoside from the flower and fruit of L. chrysantha by soxhlet extraction method, and determined the hyperoside content by RP-HPLC. The results showed that the hyperoside content had a good linear relationship with peak area in the range of 0. 300 - 1. 200 μg （r = 0. 999 6）, the average recovery rate was 99.42% （RSD = 0.313%）, The method is simple, reliable, and reproducible.

关 键 词：RP—HPLC 黄花忍冬 金丝桃苷 

分 类 号：S567.19[农业科学—中草药栽培] Q946.839[农业科学—作物学]