两种玻璃化液冻存小鼠早期囊胚的效果分析  

Study on the cryopreservation of mouses early blastocysts by two vitrification solutions

作　　者：鲁栋梁[1] 崔曙[1] 陈在贤[2] 

机构地区：[1]川北医学院附属医院泌尿外科,四川南充637000 [2]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《四川医学》2013年第3期297-299,共3页Sichuan Medical Journal

基　　金：四川省教育厅科研课题(编号:10ZB136);重庆市卫生局科研课题资助项目(编号:01-2-032)

摘　　要：目的研究玻璃化液EDS-40对冻贮小鼠早期囊胚的效果。方法①EFS-40和EDS-40玻璃化溶液的玻璃化测试。②随机将小鼠早期囊胚,先10%EG中预处理2min后分别加入EDS-40和EFS-40玻璃化溶液管并迅速投入液氮中。③各组均冻存3个月,复温后,用培养液反复洗涤后移入培养孔板培养,观察胚胎存活率,囊胚培养12h后行移植,观察妊娠率及产仔率。结果两种玻璃化溶液能达到玻璃化效果;EDS-40及EFS-40冷冻复温后的存活率分别为:75.4%、64.2%,差异有统计学意义(P<0.05);EDS-40及EFS-40组冻胚移植后的妊娠率及产仔率分别为:53.33%、23.08%,45.45%,18.18%,差异无统计学意义(P>0.05)。结论 EDS-40较EFS-40玻璃化溶液冻存小鼠早期囊胚效果更好。Objective To study the cryoprotective effect of the solution of EDS-40 and EFS-40 on mouses early blasto- cysts. Methods Test the verifying effect of EDS-40 and EFS-40 solutions. Randomly chose mice early blastoeysts to vitrify in EFS-40 or EDS-40 solutions by cryoloop and immerse into liquid nitrogen. All samples were cryopreserved 3 months. The emblyoswere rewarmed rapidly in 25 ℃ water, and agitated gently , then blastocysts were transferred into cultural medium and cultured. Then watch the survival rates. Some blastocysts were transplanted to recipients after being cultured for 12 hours . The number of pregnant recipients and young born was counted . Results Both EDS-d0 and EFS-d0 solutions had vitrifying effects. The survival rates of EDS-40 and EFS-40 were 75.4% and 64. 2% respectively and there are significant difference between them（P 〈0. 05） ;the number of pregnancy and birth integrated after vitrification cryopreservation had no significant difference between EDS-40 and EFS-40 （ P 〉 0. 05 ）. Conclusion The effect of EDS-40 solution is better than that of EFS-40 on vitrifying mice early blastocysts.

关 键 词：玻璃化技术 玻璃化溶液 胚胎冻贮 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]