DNA条形码在检疫性杂草银毛龙葵鉴定中的应用研究  

Applying DNA barcode to identify quarantine weed Solanum elaeagnifolium Cav

作　　者：张伟 范晓虹[2] 邵秀玲[3] 熊玉芬[2] 李春喜 

机构地区：[1]山东大学(威海)海洋学院,山东威海264209 [2]中国检验检疫科学研究院 [3]山东出入境检验检疫局 [4]威海出入境检验检疫局

出　　处：《植物检疫》2013年第3期60-65,共6页Plant Quarantine

基　　金：国家质检总局科技计划项目(2010IK268)

摘　　要：银毛龙葵是我国进境植物检疫性有害生物,到目前为止我国还没有关于银毛龙葵的分子检测方法。本研究收集26份茄属植物样品,包括银毛龙葵5个不同来源的个体以及它的15个近缘种,使用了3个DNA区段trnS-trnG、ndhF和waxy来探讨银毛龙葵的DNA条形码检测方法。研究结果显示银毛龙葵从其近缘种中被成功鉴定出;此外trnS-trnG、ndhF和waxy 3个片段由于具有较强的分辨力而被建议作为茄属植物补充条形码。该研究对于银毛龙葵及其他茄属植物的检疫和监测具有重要价值。Solanum elaeagnifolium Cav. is an important quarantine weed in China and other countries. To date there has been no molecular method for this species identification. In this paper, we selected 26 samples （including 5 individuals of S. elaeagnifolium and its 15 relatives）,using trnS-trnG, ndhF and waxy DNA regions to explore the utility of DNA barcode for S. elaeagnifolium identification. Our results showed that S. elaeagnifolium can be successfully discriminated from its closest relatives. In addition, the combination of the three DNA regions revealed great discriminatory power and was suggested as the supplementary barcode in Solanum plants. Our establishment of this detection method would strongly promote the quarantine of Solarium plant weeds.

关 键 词：银毛龙葵 DNA条形码 trnS-trnG基因间隔区 ndhF基因 WAXY基因 

分 类 号：S41-30[农业科学—植物保护]