固相萃取结合GC-MS系统分离分析生物体液中常见毒物药物  

COMPREHENSIVE SCREENING OF DRUGS AND TOXICANTS IN BIOLOGICAL SAMPLES USING SOLID PHASE EXTRACTION AND GC MS

作　　者：李莉[1] 周永新[1] 罗毅[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《药学学报》2000年第7期521-525,共5页Acta Pharmaceutica Sinica

摘　　要：目的　研究生物体液中 7类 47种常见毒物药物的系统分离分析方法。方法　选用通用性较好的新型紧压式三相混合填料的固相萃取柱 (SPECMulti Modal) ,采用酸性和碱性两步洗脱 ,建立同时提取血浆中几十种具不同理化性质的酸性、中性和碱性药物的系统化固相萃取方法。GC MS系统分析方法将色谱系统的保留性质和质谱系统的特征离子、谱库检索相结合实行多指标定性。利用所选的每类药物及其代谢物代表碎片离子的质量色谱 ,对几类药物及其代谢物进行筛选。结果　测定了 2 1种常见药物毒物在血浆中的绝对回收率 ,回收率均在 78%以上 ,相对标准偏差小于 7% (n =5 )。所建系统分析方法对急性中毒病人进行了定性定量分析 ,取得满意的结果 ,使方法在实际应用中得到了验证。结论　此方法系统性强、选择性好、灵敏度高 ,结果可靠。AIM To develop a method for systematic analysis of 47 toxicants and drugs in plasma. METHODS A new versatile solid phase extraction/concentrator (SPEC) plus Multi Modal disc was selected to extract the acidic, neutral and basic drugs by two different eluents, acidic ethyl acetate and basic ethyl acetate. The determinations were programmed by combining retention time, diagnostic ions and library search. Six to eight ions per category were individually selected from the mass spectra of the corresponding drugs and their metabolites. The screening was performed using reconstructed mass chromatograms of selected ions per category. RESULTS The absolute recoveries of 21 drugs from plasma exceeded 78% with relative standard deviations less than 7% at concentrations of 20 μg·mL -1 and 5 μg·mL -1 . The developed methods were applied to the detection of acute toxicants with good results. CONCLUSION These developed methods are simple, sensitive, rapid and reliable.

关 键 词：固相萃取法 GC-MS 毒物药物 生物体液 

分 类 号：R991[医药卫生—毒理学]