小鼠淋巴瘤细胞 TK 基因突变试验检测阿特拉津的诱变性  

Mutagenicity detection of atrazine by TK gene mutation test

作　　者：杜渝[1] 邹思颖[1] 张立实[1,2] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]食品安全监测与风险评估四川省重点实验室

出　　处：《现代预防医学》2013年第10期1903-1905,1913,共4页Modern Preventive Medicine

基　　金：国家自然科学基金重点项目(81030053):水产品/农产品/香料中化学污染物/产毒真菌的快速检测和食品中有害物质健康危害的安全性评价研究

摘　　要：目的了解除草剂特拉津是否具有诱变性。方法采用小鼠淋巴瘤细胞TK基因突变试验,设6.25、12.5、25、50、100μg/ml5个剂量组,不加S9条件下处理L5178Y细胞3h和12h,加S9条件下处理细胞3h,分别进行细胞毒性和突变频率的测定。结果随阿特拉津染毒剂量的增加,3种处理条件下各剂量组的相对存活率(RV)和相对悬浮增长率(RSG)均降低,突变频率增高,且有剂量反应关系;在100μg/ml剂量组L5178Y细胞tk位点突变频率与溶剂对照组自发突变频率相比差异有统计学意义。结论阿特拉津对L5178Y细胞有明显的细胞毒性和一定的遗传毒性。OBJECTIVE To evaluate the mutagenic effect of atrazine. METHODS LS178Y mouse lymphoma ceils were ex- posed to atrazine at concentrations of 6.25, 12.5, 25, 50 and 100 μg/ml for 3 hours and 12 hours without 59 metabolic activa- tion, and for 3 hours with S9 metabolic activation. Determination cytotoxicity and mutant frequency of tk locus by microwell method. RESULTS The relative suspension growth and relative viability of L5178Y cells decreased, and mutant frequency in- creased all in a dose-dependent manner as the concentration of atrazine increased in the three treatment conditions. Atrazine of 100μg/ml induced mutant frequency of tk locus in L5178Y cells increased more significantly than solvent control group in all three groups. CONCLUSION Atrazine has obvious cytotoxicity and potential mutagenic effects for L5178Y cells.

关 键 词：L5178Y细胞 TK基因突变 阿特拉津 诱变性 S9 

分 类 号：R994.1[医药卫生—毒理学]