Determination of Hesperidin in Citrus Peel by HPLC  

HPLC法测定和研究橘皮中的橙皮苷(英文)

作　　者：王宁芳[1] 

机构地区：[1]青海师范大学民族师范学院化学系,青海西宁810008

出　　处：《Agricultural Science & Technology》2013年第4期554-555,576,共3页农业科学与技术（英文版）

摘　　要：[Objective] This study aimed to measure the hesperidin content in citrus peel by high performance liquid chromatography, to provide a scientific basis for quality control and identification. [Method] The hesperidin was extracted with alkaline solution at 70 ~C and pH 6-7, and the purity of hesperidin was determined by HPLC. [Result] The formula for the regression line was Y=466,097Xq3.415 0 （r=0.999 6）, identify- ing the relationship between hesperidin concentration and peak area, and the linear range was 0.2-1.4μg. The hesperidin solution was stable within 24 h at room temperature. The average recovery rate of hesperidin was 98.41%. [Conclusion] The HPLC method is rapid, simple, and with good linear relationship, can be used for routine analysis of hesperidin.[目的]用高效液相色谱法测定橘皮中橙皮苷含量,为其质量评价和鉴定提供科学依据。[方法]在温度70℃左右,pH为6～7条件下,用碱性溶液提取橙皮苷,并采用HPLC法对提取到的橙皮苷进行纯度测定。[结果]橙皮苷浓度与峰面积之间的回归方程为Y=466.097X-0.4150(r=0.9996),线性范围为0.2-1.4μg;橙皮苷样品溶液室温下放置24h稳定良好;橙皮苷的平均回收率为98.41%。[结论]HPLC法快速、简便、线性关系良好,可以作为橙皮苷的一种常规分析方法。

关 键 词：Citrus peel HESPERIDIN High performance liquid chromatography （HPLC） 

分 类 号：O657.72[理学—分析化学] S666.2[理学—化学]