Gene Expression Profile of IGF-Ⅰ mRNA and Its Correlation with Body Weight in Different Goose Breeds  

不同鹅种IGF-Ⅰ mRNA的表达特异性及其与体重的相关性分析(英文)

作　　者：汤青萍[1] 宋迟[1] 章双杰[1] 胡艳[1] 邹剑敏[1] 

机构地区：[1]江苏省家禽科学研究所,江苏省家禽遗传育种重点实验室,江苏扬州225003

出　　处：《Agricultural Science & Technology》2013年第4期568-572,共5页农业科学与技术（英文版）

基　　金：Supported by the Key Science and Technology Research and Development Program of Jiangsu Province(BE2010370);the Natural Science Foundation of Jiangsu Province (BK2001432);Earmarked Fund for China Agriculture Research System(nycytx-42-G1)~~

摘　　要：[Objective] This study aimed to investigate the expression of IGF-I mRNA in breast and leg muscles of goose at the embryonic period and early growth stage, as well as the correlations between IGF-I mRNA expression and goose body weight. [Method] The expression of IGF-I mRNA in breast and leg muscles of 23, 25, 27, 29-embryo days and 0, 2, 4, 6, 8, 10, 12 week old geese of Taihu and Wanxi breeds was analyzed with multiplex competitive fluorescent-PCR method. [Result] Goose IGF-I mRNA was detected in breast and leg muscles of 23-embryo days of the both goose breeds. There were some differences in the IGF-I mRNA expression profiles between the two goose breeds during development stage. The body weight of Taihu goose was positively related with the IGF-I mRNA expression in leg muscle, and the body weight of Wanxi goose was positively related with IGF-I mRNA level in breast muscle. [Conclusion] IGF-I mRNA in the muscle of goose might play an important role in early development stage.[目的]研究不同鹅种胚胎期和生长早期胸肌和腿肌中IGF-Ⅰ mRNA的表达规律及其与体重的相关性分析。[方法]选择生长速度差异较大的太湖鹅和皖西白鹅为试验模型,采用多重荧光竞争性PCR方法研究鹅23、25、27、29胚龄和0、2、4、6、8、10、12周龄胸肌和腿肌中IGF-Ⅰ mRNA的表达规律,并对其与体重的相关性进行比较。[结果]IGF-Ⅰ mRNA在鹅胚胎期胸、腿肌中都有表达,且表达早于23胚龄;不同品种鹅早期肌肉组织中IGF-Ⅰ mRNA的表达发育模式存在部分差异;太湖鹅体重与腿肌中IGF-ⅠmRNA的表达呈显著正相关,皖西白鹅体重与胸肌中IGF-Ⅰ mRNA的表达呈显著正相关。[结论]肌肉IGF-Ⅰ mRNA的表达可能在鹅早期生长发育过程中发挥着重要作用。

关 键 词：GOOSE Muscle IGF-I mRNA Body weight 

分 类 号：S835[农业科学—畜牧学]