Preservation and Conidia Culture Technique of Ustilaginoidea virens  

水稻稻曲病菌保存与培养技术研究(英文)

作　　者：何海永 Sopone WONGKAEW Natthiya BUENSANTEAI 杨学辉 陈小均 吴石平 谭清群 王莉爽 袁洁 

机构地区：[1]贵州省植物保护研究所,贵州贵阳550006 [2]苏兰拉里理工大学农业技术学院作物生产系

出　　处：《Agricultural Science & Technology》2013年第4期624-626,共3页农业科学与技术（英文版）

基　　金：Supported by the Programs of Guizhou Academy of Agricultural Sciences in China [No. QianNongKeYuan(2010) 033 and No.QianNongKeHe 07016];the Programs of Science and Technology Department of Guizhou Province [No.QianKeHe J(2009) 2103,and No.QianKeHe NY(2012) 3031];Science and Technology Project of Guizhou Province [QKH Major Project(2012) 6012]~~

摘　　要：[Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition were tested by shaking cul- ture at 150 r/min. [Result] Among the six methods studied, the periodic transfer and paraffin oil overlay were suitable, and the fungus could survive more than 12 months. The conidia concentration went up to 7.25×10^7 conidia/ml after 9 d in the PSB liquid medium, indicating that PSB was the best medium for conidia production. [Conclusion] This study provides a theoretical basis for the preservation and conidia production of U. virens.[目标]研究稻曲病菌分生孢子培养和病菌呆存方法。[方法]每间隔3个月对6种不同保存方法的稻曲病菌菌株的存活力进行检验;通过振荡(150r/min)的方法,对7种不同组成的液体培养基的产孢能力进行了研究。[结果]在6种不同保存方法中,周期地转移和石蜡油保存是比较适合的方法,病原菌能存活超过12个月以上;稻曲病菌在PSB培养基中被培养9d后,分生孢子浓度能达到7.25×107个/ml。

关 键 词：PRESERVATION Conidia culture Ustilaginoidea virens 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]