维甲酸诱导分化对SH-SY5Y细胞阿片受体表达的影响  

Effect of retinoic acid-induced differentiation on expression of opioid receptors in SH-SY5Y cells

作　　者：边佳明[1] 吴宁[2] 李锦[2] 

机构地区：[1]北京军区总医院药理科,北京100700 [2]军事医学科学院,毒物药物研究所,北京100850

出　　处：《中国药理学通报》2013年第5期697-699,共3页Chinese Pharmacological Bulletin

基　　金：973项目(Grant No 2009CB522008);国家自然科学基金项目(Grant No 30772563);北京市自然科学基金项目(Grant No 7082075)的支持

摘　　要：目的研究维甲酸诱导SH-SY5Y细胞分化后对阿片受体表达的影响。方法 1×10-5mol.L-1维甲酸诱导SH-SY5Y细胞分化6天,[3H-diprenorphine]放射性配体受体结合试验确定阿片受体表达量。结果维甲酸诱导分化6天后,与对照细胞比较,SH-SY5Y细胞突起增多增长类似轴突,且细胞间形成明显神经纤维网络,细胞生长明显减慢,阿片受体表达提高1.6倍以上。结论维甲酸明显诱导SH-SY5Y细胞向神经元分化,且分化后细胞阿片受体表达明显上调。Aim To explore the effect of retinoic acid （RA） -induced differentiation on the expression of opioid receptors in SH-SY5Y cells. Methods SH-SYSY cells were induced to differentiation by 1×10-5mol.L-1 of RA, and the amount of opioid receptors was determined by [ ^3 H-diprenorphine ] binding assay. Resuits Compared with control cells, 6 days of RA induction resulted in remarkable differentiation towards neuron-like cells with longer neurite, formation of network, decreased growth rate and upregulated expression of opioid receptors by 1.6 folds. Conclusion RA induces a significant differentiation of SH-SY5Y ceils towards neuron-like, and opioid receptors are upregulated remarkably.

关 键 词：阿片受体 维甲酸 表达 分化 SH-SY5Y细胞 神经元 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R392.11[医药卫生—基础医学]