两种方法制备胶体金及其免疫标记的比较研究  

Study on Preparation of Colloidal Gold by Two Methods and Comparison of Immune Markers

作　　者：张晓慈[1] 杨孝朴[1] 祁光宇[1,2] 

机构地区：[1]甘肃农业大学,甘肃兰州730070 [2]中农威特生物科技股份有限公司,甘肃兰州730046

出　　处：《中国饲料添加剂》2013年第5期13-16,共4页China Feed Additive

摘　　要：为寻找-种适合口蹄疫Asia I抗体标记的胶体金，本研究采用柠檬酸三钠和柠檬酸三钠－鞣酸法分别还原氯金酸溶液，制备纳米胶体金，并分别对制得的胶体金粒子大小、粒径分布情况、稳定性及微观形貌进行了分析，同时进行口蹄疫抗体标记。结果显示，柠檬酸三钠-鞣酸法制备纳米胶体金适合口蹄疫AsiaI抗体的标记，柠檬酸三钠－鞣酸法制备胶体金较稳定．标记的试纸条灵敏度耍高于柠檬酸三钠法。To search for a suitable colloidal gold, in this study, sodium citrate and sodium citrate - tannic acid were used to restore gold chloride acid solution respectively, and the nano colloidal gold was prepared. The properties of particle size, size distribution, stability and morphology of colloidal gold were analyzed, at the same time anti - FMDV type Asia I antibody mark- ers were studied. The results showed that, the preparation of colloidal gold by sodium citrate - tannic acid is more stable, and more suitable for anti -FMDV type Asia I antibody labeled, and the marked test strip sensitivity by sodium citrate - tannic acid is higher than that by sodium cit- rate method.

关 键 词：柠檬酸三钠 柠檬酸三钠-鞣酸法 胶体金 免疫层析 蛋白标记 

分 类 号：S945.26[农业科学—水产养殖]