黄芪三仙汤干预成骨细胞护骨素及护骨素配体的表达  

Huangqi Sanxian decoction is involved in gene regulation of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts

作　　者：黎晓敏 卢昌均[2] 周艳芳[1] 周志昆[1] 冯鑫[1] 郑丽华[1] 

机构地区：[1]广东医学院药学院,广东省东莞市523808 [2]广西柳州市中医院,广西壮族自治区柳州市545001

出　　处：《中国组织工程研究》2013年第11期1939-1945,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2006B35601005)~~

摘　　要：背景:长期临床研究表明黄芪三仙汤对骨质疏松症有一定的治疗作用,基础实验研究也表明黄芪三仙汤能改善实验动物的生物力学指标。在此基础上实验拟从细胞分子水平上探讨黄芪三仙汤对成骨细胞的相关作用。目的:观察黄芪三仙汤对体外培养成骨细胞护骨素、护骨素配体基因调节的作用,探讨黄芪三仙汤治疗骨质疏松的作用机制。方法:制备黄芪三仙汤和仙灵骨葆胶囊含药血清及空白血清,对体外培养新生SD大鼠成骨细胞进行干预,应用细胞形态观察、MTT、Western blot分析法及蛋白浓度测定法,观察各含药血清对体外培养成骨细胞的增殖及成骨细胞护骨素、护骨素配体表达的影响。结果与结论:经药物干预后成骨细胞变为长梭形、条索状,融合成片,分界较为模糊,细胞生长较密集。黄芪三仙汤可明显促进成骨细胞护骨素蛋白浓度的提高(P<0.01),同时也提高了护骨素配体蛋白浓度(P<0.01),而护骨素/护骨素配体浓度比例水平明显升高(P<0.01)。结果可见黄芪三仙汤可能是通过调节成骨细胞分化调控因子护骨素、护骨素配体的表达来调节成骨细胞的增长,从而实现其对骨质疏松症的治疗作用。BACKGROUND： Long-term clinical research has demonstrated that Huangqi Sanxian decoction exerts a certain curative effect on osteoporosis. In addition, experimental studies have also showed that Huangqi Sanxian decoction can improve the biomechanical indexes in experimental animals. The present study aimed to investigate the effect of Huangqi Sanxian decoction on osteoblasts in the cellular level. OBJECTIVE： To observe the effects of Huangqi Sanxian decoction on gene regulation of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro and to investigate the action mechanism underlying the curative effect of Huangqi Sanxian decoction on osteoporosis. METHODS： Huangqi Sanxian decoction, Xianlinggubao capsule containing serum and blank serum were prepared to intervening osteoblasts from newborn Sprague-Dawley rats cultured in vitro. Cellular morphology was observed, and western blot analysis and determination of protein concentration were used to observe the effects of drug-containing serum on osteoblast proliferation, gene expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro. RESULTS AND CONCLUSION： After drug intervention, osteoblasts were spindle-shaped and trabs-shaped, which were concentrated and confluent with undefined boundary. Huangqi Sanxian decoction could increased the protein expression of osteoprotegerin in osteoblasts （P 〈 0.01）, and also increased the protein expression of receptor activator of nuclear factor kappa B ligand （P 〈 0.01 ） to increase ratio of osteoprotegerin/receptor activator of nuclear factor kappa B ligand （P 〈 0.01 ）. Huangqi Sanxian decoction can achieve the therapeutic effect on osteoporosis through regulating the expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand.

关 键 词：组织构建 骨组织构建 黄芪三仙汤 补肾活血 成骨细胞 护骨素 护骨素配体 骨质疏松 细胞培养 破骨细胞 骨吸收 骨形成 省级基金 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]