Ⅱ型胶原注射骨关节炎时C-反应蛋白及类风湿因子的表达  

Expression of rheumatoid factor and C-reactive protein during type Ⅱ collagen treatment of osteoarthritis

作　　者：雷静 钟翠红 陈淡嫦 黄雷 

机构地区：[1]广州创尔生物技术有限公司研发中心,广东省广州市510663 [2]广州凯恒科塑有限公司,广东省广州市510180

出　　处：《中国组织工程研究》2013年第11期2007-2012,共6页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目-社会发展专项(2009B030803060)~~

摘　　要：背景:Ⅱ型胶原和硫酸软骨素是关节软骨的重要组成成分,不仅具有保护软骨的作用,还能抑制关节炎的病变。目的:通过检测类风湿因子和C-反应蛋白的表达评价Ⅱ型胶原及Ⅱ型胶原复合硫酸软骨素注射对骨性关节炎的疗效。方法:采用改良Hulth法制作大鼠膝关节骨性关节炎模型,造模后模型组关节腔注射生理盐水、透明质酸钠组给予透明质酸钠凝胶注射、Ⅱ型胶原组给予单纯Ⅱ型胶原注射、Ⅱ型胶原复合硫酸软骨素组给予Ⅱ型胶原复合硫酸软骨素注射治疗。各组动物于术后进行日常观察、称质量、血清抗体及组织病理检查。结果与结论:造模后模型组大鼠血类风湿因子和C-反应蛋白水平均有所升高,炎症加剧;而与模型组相比,各给药组大鼠类风湿因子和C-反应蛋白水平均有所下降,炎症有所减轻,其中Ⅱ型胶原复合硫酸软骨素组大鼠血清中类风湿因子水平显著下降(P<0.01)。结果显示Ⅱ型胶原与透明质酸钠均具有预防和治疗骨关节炎的作用,能缓解骨关节炎病变,Ⅱ型胶原联合硫酸软骨素的疗效更明显。BACKGROUND： Type Ⅱ collagen and chondroitin sulfate are the major components of articular cartilage. They cannot only protect the cartilage, but also inhibit pathology of osteoarthritis. OBJECTIVE： To evaluate the curative effect of type Ⅱ collagen injection and type Ⅱ collagen- chondroitin sulfate composite injection on osteoarthritis by detecting the expression of rheumatoid factor and C-reactive protein. METHODS： A rat model of knee osteoarthritis was established by using improved Hulth＇s method. The treatment was performed via injection into the articular cavity （treatment group A： type Ⅱ collagen injection treatment group B： type Ⅱ collagen-chondroitin sulfate composite injection; positive control： sodiumhyaluronate; model control： physiological saline）. After operation, rats in each group were characterized with daily observation, weighing, serum antibody test and histological observation. RESULTS AND CONCLUSION： After treatment, the content of rheumatoid factor and C-reactive protein in rats serum of model group was increased, and inflammation was aggravated; while compared with the model group the content of rheumatoid factor and C-reactive protein in the other groups, especially in the treatment group B, were lower and the inflammation was eased （P 〈 0.01）. The findings show that both type Ⅱ collagen and sodium hyaluronate can prevent and treat osteoarthritis effectively. The therapeutic effect of type Ⅱ collagen combined with chondroitin sulfate is better.

关 键 词：组织构建 组织构建基础实验 Ⅱ型胶原 硫酸软骨素 骨性关节炎 类风湿因子 C-反应蛋白 透明 质酸钠 动物模型 改良Hulth法 炎症 软骨病变 省级基金 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]