人膝骨性关节炎滑膜中表达的骨桥蛋白  

Expression of osteopontin in the articular synovium of human knee osteoarthritis

作　　者：肖盛世[1,2] 高曙光[1,3] 徐迈[1,3] 张凯[1,3] 朱鹤远[1,3] 宋扬[1,3] 杨阳[1,3] 雷光华[1,3] 

机构地区：[1]中南大学湘雅医院骨科,湖南省长沙市410008 [2]株洲市一医院创伤骨科,湖南省株洲市412000 [3]中南大学湘雅医院骨科研究所,湖南省长沙市410008

出　　处：《中国组织工程研究》2013年第11期2013-2019,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目([2012]81272034/H0609);国家自然科学基金青年科学基金资助项目([2012]081201420/H0609);湖南省发展与改革委员会资助项目([2007]896);湖南省自然科学基金重点项目(09JJ3048)~~

摘　　要：背景:目前研究显示骨桥蛋白与骨性关节炎关节软骨退变密切相关,但骨桥蛋白与骨性关节炎滑膜病变是否相关,仍少见报道。目的:研究骨桥蛋白在原发性骨性关节炎发生与发展过程中的作用。方法:选取膝骨性关节炎患者关节滑膜标本与下肢外伤患者作对照,根据综合评分法进行分组,采用免疫组化方法检测关节滑膜中骨桥蛋白水平,比较不同程度骨性关节炎组膝关节滑膜中骨桥蛋白的差异,同时比较标本滑膜衬里层和衬里下层骨桥蛋白的水平。结果与结论:骨性关节炎组织中骨桥蛋白阳性呈黄色、棕黄色、棕褐色表达,病变程度越重,颜色表达越深。骨性关节炎组滑膜中骨桥蛋白明显高于对照组,差异有显著性意义(P<0.05),且随着骨性关节炎病情加重,骨桥蛋白的表达逐渐增多,呈正相关(ρ=0.663,P<0.01)。但滑膜衬里层和衬里下层骨桥蛋白的水平差异无显著性意义(P>0.05)。说明骨桥蛋白可能与骨性关节炎的发病有关。BACKGROUND： Studies have shown that osteopontin is closely related to cartilage degeneration ofosteoarthritis, but the osteopontin expression in the synovium and their relations to osteoarthritis severity are st rarely reported. OBJECTIVE： To investigate osteopontin effects on the occurrence and development of primary knee osteoarthritis. METHODS： Synovium samples from patients with knee osteoarthritis and low-limb trauma controls were enrolled in this study. The comprehensive score method was taken for grouping. Osteopontin levels in the synovium were measured using immunohistochemical method. The differences among groups were compared. The level of osteopontin between the synovial lining layer and the sub-lining layer was also compared. RESULTS AND CONCLUSION： In the tissue of osteoarthritis, positive expression of osteopontin was found, showing yellow, burry, and brown in color. Compared to the controls, osteoarthritis patients had higher osteopontin level in the synovium （P 〈 0.05）. In addition, subsequent analysis showed that osteopontin levels in the synovium were significantly correlated with severity of disease （,o=0.663, P 〈 0.01 ）. There was no significant difference in the osteopontin level between the synovial lining layer and the sub-lining layer （P 〉 0.05）. These data suggest that osteopontin may be associated with the pathogenesis of osteoarthritis.

关 键 词：组织构建 组织构建基础实验 骨性关节炎 骨桥蛋白 滑膜病 关节软骨 发病机制 滑膜 衬里层 衬里下层 关节软骨退变 免疫组化 国家自然科学基金 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]