帕金森大鼠消化道组织中蛋白质NHERF家族和CAL的表达变化  

Change of NHERF family protein and CAL protein in Parkinson's rats gastrointestinal tissue

作　　者：史小翠[1] 冯小燕[2] 宋然[1] 杨龙燕[1] 贺俊崎[1] 

机构地区：[1]首都医科大学基础医学院生物化学与分子生物学系,北京100069 [2]首都医科大学基础医学院生理学系,北京100069

出　　处：《首都医科大学学报》2013年第2期232-238,共7页Journal of Capital Medical University

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB94480101);国家自然科学基金(81272887)~~

摘　　要：目的研究NHERF家族蛋白和CAL蛋白在帕金森病(Parkinson’s disease,PD)消化系统中的表达,探讨帕金森病消化系统功能紊乱的可能机制。方法用6-羟多巴(6-hydroxydopamine,6-OHDA)损毁大鼠中枢黑质多巴胺能神经元,建立帕金森大鼠模型。采用蛋白免疫印迹法(Western blotting),检测NHERF1﹑NHERF2﹑NHERF3﹑NHERF4以及CAL在6-OHDA处理模型大鼠胃体﹑十二指肠﹑结肠中表达的情况。结果成功造模后,利用Western blotting的方法,发现帕金森模型大鼠胃体PDZK1和CAL蛋白表达水平上调,帕金森模型大鼠十二指肠NHERF1、NHERF2、PDZK1表达水平高于正常组,以及帕金森模型大鼠结肠NHERF1、PDZK1、CAL表达水平也高于正常组。结论帕金森大鼠消化道组织中NHERF家族和CAL蛋白表达的上调,为研究帕金森患者胃肠道功能紊乱方面的机制提供线索。Objective To study the expression of NHERF family protein and CAL protein in the digestive system of Parkinson＇ s disease, and investigate the mechanism of the dysfunction of digestive system in Parkinson＇s disease. Methods Snbstantia nigra dopaminergic neurons of the SD rats were damaged by injecting 6-hydroxydopamine （6-OHDA） in the brain substantia nigra. Western blotting analysis for the expression of NHERF family protein and CAL protein in the gastry, duodenum and colon of the control and 6- OHDA treated rats were performed. Results The Western blotting results showed that the protein level of PDZK1 and CAL in the gastric corpus was enhanced in 6-OHDA treated rats, the protein level of NHERF1, NHERF2 and PDZK1 in the duodenum was increased in 6- OHDA treated rats, the protein level of NHERF1, PDZK1 and CAL in the colon was enhanced in 6-OHDA treated rats. Conclusion The upregulation of NHERF family protein and CAL protein may contribute to the dysfunction of digestive system in 6-OHDA treated rats . These results provide a strong evidence for further studying the mechanisms of digestive system dysfunction in Parkinson＇ s disease.

关 键 词：NHERF家族蛋白 CAL 6-羟多巴处理模型大鼠 胃 十二指肠 结肠 

分 类 号：R392.11[医药卫生—免疫学]