高效液相色谱法同时测定中毒患者血清中5种精神活性物质的浓度  

Simultaneous determination of five psychoactive drugs in overdosed patients serum by high performance liquid chromatography

作　　者：果伟[1] 赵晔[1] 郭桂欣[1] 张军[1] 孙川[1] 荣璋[1] 段晓霞 李文标[1] 翟屹民[1] 

机构地区：[1]首都医科大学附属北京安定医院临床精神药理实验室,北京100088 [2]太原精神病医院检验科,山西太原030045

出　　处：《首都医科大学学报》2013年第2期270-274,共5页Journal of Capital Medical University

基　　金：北京市委组织部优秀人才培养专项基金(2004);首都医学科研发展基金(2009-3135)~~

摘　　要：目的建立可同时测定人血清中利培酮、9-羟利培酮、氯氮平、去甲氯氮平和瑞波西汀5种药物浓度的高效液相色谱-紫外检测法,并将该方法用于临床急性精神药物中毒患者的血液样本分析。方法以乙醚为溶剂进行一步液液萃取,待测物与内标帕罗西汀在Inertsil ODS-3柱,乙腈∶甲醇∶正丁胺∶冰醋酸∶去离子水(20∶2.87∶1.07∶1∶34.29)条件下可以基线分离。线性范围100～1 600 ng/mL,最低检测限25μg/L(S/N=10)。结果本方法批内精密度为3.1%～15.6%,批间精密度为5.7%～18.4%;批内准确度为0.1%～8.7%,批间准确度为0.3%～13.3%。常见精神科药物不干扰待测药物和内标。结论本方法简单、特异性好,可应用于急诊患者药物中毒检测。Objective A high performance liquid chromatography（HPLC） method with ultraviolet（UV） detection（230 nm） was developed and validated for the quantification of 5 drugs, Risperidone, clozapine, reboxetine, 9-hydroxy Risperidone and norclozapine, in human serum. Methods Following a one-step liquid-liquid extraction with diethyl ether, the analyte and internal standard（paroxetine） were separated using an isocratic mobile phase consisted of acetonitrile ：methanol ：butylamine ：acetic acid ：deionized water = 20：2.9：1.1： 1：34.3 on a reverse phase Inertsil C1s-3 column. Five drugs and IS were separated well under the chromatographic condition. The calibration curve was verified over the range of 100 - 1 600 μg,/L and limit of detection（LOD） was 25 μg/L（ S/N = 10）. Results The imprecision of within and between batches were 3.1% - 15.6% and 5.7% -18.4%, respectively. The accuracy of within and between batches were 0. 1% - 8.7% and 0.3% - 13.3%. Commonly used psychotropic drugs did not interfere with the drugs and internal standard. Conclusion This method was found to be rapid, simple, accurate, sensitive and more suitable for rapid qualitative and quantitative determination of drugs in the serum of overdosed patients.

关 键 词：过量中毒 液液萃取 高效液相色谱 

分 类 号：R971.41[医药卫生—药品]