盐酸纳美芬对大鼠颅脑创伤后Survivin表达的影响  

Effect of Nalmefene on survivin expression after traumatic brain injury in rats

作　　者：张兴超 张绍政[2] 彭龙锋[2] 曲绍霞[2] 姜京超 高培龙[3] 

机构地区：[1]青岛开发区第一人民医院神经外科,山东266555 [2]烟台市莱阳中心医院神经外科,山东265200 [3]兰州大学第一附属医院神经外科,甘肃730050

出　　处：《创伤外科杂志》2013年第3期255-258,共4页Journal of Traumatic Surgery

基　　金：甘肃省科技厅(技术研究与开发专项计划)项目(1004tcya026)

摘　　要：目的观察盐酸纳美芬对大鼠颅脑创伤后生存素(Survivin)表达的影响,探讨其脑保护机制。方法 78只Wistar大鼠随机分为假手术组(n=6)、颅脑创伤组(n=36)及纳美芬治疗组(n=36)。采用自由落体法建立大鼠颅脑创伤模型,应用Epics XL流式细胞仪检测细胞凋亡情况并应用免疫组织化学法检测Survivin和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达变化。结果与假手术组相比,颅脑创伤组细胞凋亡率及Survivin、Caspase-3蛋白表达水平明显升高。盐酸纳美芬可促进Survivin表达,并通过相关性分析,其表达水平和Caspase-3表达具有负相关性。结论盐酸纳美芬可能通过增加抗凋亡蛋白Survivin表达来抑制Caspase-3激活,减少颅脑创伤后细胞凋亡,发挥神经保护作用。Objective To investigate the effect of Nalmefene on survivin expression in rats after traumatic brain injury （ TBI）, and explore the mechanisms of neuro-protection by Nalmefene. Methods Seventy-eight adult Wistar rats were randomly divided into sham-operation group （ n = 6 ）, traumatic brain injury （TBI） group { n = 36 ） and Nalmefene group （ n = 36）. The experimental TBI model was established by Feeney＇ s method. Samples were obtained after injury for measuring the cells apoptosis by Epics XL Flow Cytometer and inspecting the expression of survivin and caspase - 3 proteins by an iinmunochemical method. Results Compared with the sham-operation group, the apoptosis of cells and expression of survivin and caspase-3 proteins were significantly increased in the in- jured brain after TBI. Nalmefene significantly increased the expression of survivin, which was negatively correlated with the activity of caspase-3 according to the correlation analysis. Conclusion Increasing the expression of sur- vivin by nalmefene might be one of the mechanisms in regulating caspase-3 level and inhibiting neuronal apoptosis in cortex and exerting protective function to neurons.

关 键 词：颅脑损伤 纳美芬 生存素 半胱氨酸天冬氨酸蛋白酶-3 凋亡 大鼠 

分 类 号：R651.1[医药卫生—外科学]