不同来源金黄色葡萄球菌Panton—Valentine杀白细胞素基因的表达及其与agrA表达相关性研究  

The expression of Panton-Valentine leukocidin gene and its correlation with agrA among different clinical isolates of Staphylococcus aureus

作　　者：刘莹[1] 李挺建[1] 卢园园[1] 王良兴[1] 余方友 

机构地区：[1]温州医学院附属第一医院呼吸科,325000 [2]检验科

出　　处：《中华检验医学杂志》2013年第4期313-317,共5页Chinese Journal of Laboratory Medicine

基　　金：基金项目：国家自然科学基金资助项目（I-12002/81271906）;浙江省自然科学基金资助项目（Y2100716）

摘　　要：目的研究不同标本分离的金黄色葡萄球菌临床分离株Panton-Valentine杀白细胞素（PVL）的表达及与agrA表达的相关性。方法收集2003年1月至2010年12月4家医院从临床各种标本中分离的金黄色葡萄球菌，所有菌株均为非重复株。采用普通PCR和DNA测序检测PVL基因。采用实时荧光定量PCR检测lukSmRNA和agrAmRNA的量。结果经过PCR扩增及测序共筛检出96株PVL阳性的金黄色葡萄球菌，其中58株为医院获得株，28株为社区获得株，10株由于临床资料不全不能区分；54株分离白血液标本，33株分离自脓液标本及9株分离白痰液标本；67株分离自成人的临床标本，29株分离自儿童的临床标本。分离自脓液标本和血液标本的菌株lukSmRNA的相对表达量的中位数分别为1．500和0．818，分离自脓液标本菌株的lukSmRNA的表达量明显高于分离白血液标本菌株的量（U=634，P=0．025）；分离自儿童和成人的菌株lukSmRNA的相对表达量的中位数分别为1．292和0．540，儿童分离株的lukSmRNA的表达量明显高于成人分离株的量（U=660，P=0．013）；社区分离株和医院分离株lukSmRNA的相对表达量的中位数分别为1．034和0．536，社区获得株的lukSmRNA的表达量明显高于医院获得株的量（U：338，P=0．012）。在总的分离株中，lukSmRNA与agrAmRNA的表达量存在正相关性（r=0．592，P〈0.01）；分离自脓液标本菌株的lukSmRNA与agrAmRNA相关系数较高（r=0．810，P〈0．01），但分离自血液标本菌株的两者相关性系数较低（r=0．543，P〈0．叭）。分离自儿童标本菌株的lukSmRNA与agrAmRNA量之间相关性更高，为0．804（P〈0．01），而分离自成人标本菌株的两者相关系数为0．476（P〈0．01）。社区获得株与医院获得株两者的相关系数分别为0．767（P〈0．01）和0．556（P〈0．01）。结论不同的感染类型luks／F—PV基因的表达不同，agr系统可能对lukS�Objective To investigate the correlation between expression of Panton-Valentine leukocidin gene and accessory gene regulator among different clinical isolates of Staphylococcus aureus. Methods All non-duplicate Staphylococcus aureus clinical isolates were isolated from various clinical specimens of the patients at 4 hospitals from January 2003 to December 2010. Panton-Valentine leukocidin genes among Staphylococcus aureus clinical isolates were detected by PCR and DNA sequencing. The expressions of lukS-PV and agrA were determined by real-time PCR. Results Ninty-six S. aureus isolates including 58 hospital-acquired and 28 community-acquired isolates were positive for PVL genes, among which 54 from blood, 33 from pus and 9 from sputum. Ten isolates cannot be classified due tolack of information. Sixty-seven and 29 PVL-positive isolates were isolated from the specimens of adults and children. The median relative quantities of lukSmRNA of the isolates from pus and blood were 1. 500 and O. 818. The quantity of lukSmRNA among the isolates from pus was significantly higher than that from blood （ U = 634 ,P = 0. 025 ）. The median relative quantities of lukSmRNA of the isolates from children and adults were 1. 292 and 0. 540, respectively. The quantity of lukSmRNA among the isolates from children was significantly higher than that from adults （ U = 660 ,P = 0. 013 ）. The median relative quantities of lukSmRNA among community-acquired and hospital-acquired isolates were 1. 034 and 0. 536, respectively. The quantity of lukSmRNA among community-acquired isolates was significantly higher than that from hospital-acquired isolates （ U = 338, P = o. 012 ）. The correlation coefficients between lukSmRNA and agrAmRNA of total isolates, pus isolates and blood isolates were 0.592 （P 〈0,01）, 0. 810 （P 〈0.01） and 0,543 （P 〈 0. 0l ）, respectively. While the correlation coefficients of those among the isolates from children and adults were 0. 804 （P 〈0. 01 ） and 0. 476 （P 〈0. 01 ）. The correlati

关 键 词：葡萄球菌 金黄色 细菌毒素类 外毒素类 杀白细胞素类 细菌蛋白质类 

分 类 号：R378.11[医药卫生—病原生物学]