人类免疫缺陷病毒-1单纯及合并丙型肝炎病毒感染中人类免疫缺陷病毒-1特异性T淋巴细胞免疫应答  

Immune response of human immunodeficiency virus-1 specific T lymphocytes in human immunodeficiency virus-1 monoinfected or human immunodeficiency virus-1/hepaUtis C virus coinfected individuals

作　　者：唐漾波[1] 占晗琳 曹孟丽 赵稳 平岖[1] 

机构地区：[1]广州市第八人民医院传染病研究所,510060 [2]江西省鹰潭市人民医院内科 [3]深圳市继续教育中心 [4]广州市白云区红十字会医院内科

出　　处：《中华传染病杂志》2013年第4期216-220,共5页Chinese Journal of Infectious Diseases

摘　　要：目的了解单纯感染HIV-1及合并感染HCV时HIV-1特异性T淋巴细胞免疫应答的特点。方法单纯HIV-1感染者26例和HIV-1／HCV合并感染者23例，采用免疫磁珠分选技术从外周血单个核细胞（PBMC）中分选出CD4＋T淋巴细胞及CD8＋T淋巴细胞，采用酶联免疫斑点测定（ELISPOT），以HIV-1P24编码区的氨基酸序列人工合成12个重叠肽段组成的肽段库作为特异性抗原表位，测定CD4＋T淋巴细胞、CD8＋T淋巴细胞及PBMC的IFN-7分泌细胞频数；实时荧光定量PCR检测HIV-1RNA。组间比较采用单因素方差分析及Mann-WhitneyU检验，Spearman评价组间相关性。结果单纯HIV-1感染者的HIV-1抗原特异性CD4＋T淋巴细胞中位数为25斑点形成细胞（SFC）／10。细胞，显著低于CD8＋T淋巴细胞的38SFC／10。细胞（F=4．592，P=0．037）及PBMC的53SFC／10^6细胞（F=5．436，P=0．025）。HIV-1／HCV合并感染者的HIV-1抗原特异性CD4＋T淋巴细胞（中位数为5SFC／10^6细胞，Z=2．432，P=0．015）、CD8＋T淋巴细胞（中位数为5SFC／10^6细胞，Z=-1．996，P=0．046）及PBMC（中位数为10SFC／10。细胞，Z=2．306，P=0．021）的应答频数显著低于单纯HIV-1感染者。结论在HIV-1感染中，CD4＋T淋巴细胞能对HIV-1产生-定的特异性免疫应答，但是其强度弱于CD8＋T淋巴细胞；合并HCV感染可能体HIV-1臆诳者的H1V-1特异性T淋巴细胞应答强度下调。Objective To investigate the features of immune response of human immunodeficiency virus type-1 （HIV-1） antigen specific T lymphocytes in HIV-1 monoinfected or HIV-1/hepatitis C virus （HCV） coinfected individuals. Methods Twenty-six HIV-1 monoinfected and 23 HIV-I/HCV coinfected individuals were enrolled. Immunomagnetic microbeads were used to isolate T lymphocyte subpopulation CD4＋ T cells and CD8＋ T cells from human peripheral blood mononuclear cells （PBMC）. Frequencies of interferon-γ （IFN-γ） secreting cells of CD4＋, CD8＋T lymphocytes and PBMC stimulated by a peptide pool containing 12 overlapping peptides in HIV-1 P24 from 49 patients were assessed by enzyme-linked immunospot （ELISPOT） assay. HIV-1 RNA levelsof these patients were also detected by real-time fluorescence quantitative polymerase chain reaction. The data were compared by one-way ANOVA and Mann-Whitney U test, and Spearman test was used for correlation analysis. Results Frequencies of HIV-1 antigen specific CD4＋ T lymphocytes [median =25 spot-forming cells （SFC）/10^6 cells] were significantly lower than those of CDS＋T lymphocytes （median=38 SFC/106 cells, F=4. 592, P= 0. 037） and PBMC （median= 53 SFC/10^6 cells, F= 5. 436, P= 0. 025） in HIV-1 monoinfected group. Frequencies of HIV-1 antigen specific CD4＋T lymphocytes （median=5 SFC/10^6 ceils, Z=-2. 432, P=0. 015）, CD8＋T lymphoeytes （median= 5 SFC/106 cells, Z=-1. 996, P=0. 046） and PBMC （median=10 SFC/10^6 cells, Z=-2. 306, P= 0. 021） in HIV-1/HCV coinfected group were significantly lower than those in HIV-1 monoinfected group. Conclusions In HIV-1 infection, antigen specific immune response of CD4＋ T cells can be activated, but weaker than that of CD8＋ T cells. Co-infection with HCV might down-regulate the responses of HIV-1 antigen specific T lymphocytes in HIV-1 infected individuals.

关 键 词：HIV-1 肝炎病毒属 重叠感染 CD4阳性T淋巴细胞 CD8阳性T淋巴细胞 酶联免疫斑点测定 

分 类 号：R51[医药卫生—内科学]