中药复方对骨髓抑制小鼠血液生理指标及细胞因子的影响  

Effects of Chinese herbal compound on the blood physiological parameters and cytokines of myelosuppressive mice

作　　者：左之才[1] 甘梦[1] 崔恒敏[1] 彭西[1] 王娅[1] 邓俊良[1] 徐志文[1] 

机构地区：[1]四川农业大学动物医学院环境公害与动物疾病四川省高校重点实验室动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医科学》2013年第5期537-544,共8页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

摘　　要：通过腹腔注射环磷酰胺建立骨髓抑制小鼠模型,采用血细胞自动分析仪测定模型小鼠及使用中药复方(剂量分别为20、10和5g/kg)、黄芪多糖(66mg/kg)3d和7d小鼠的血液生理指标,用ELISA方法测定血清中白介素-3、白介素-6和促红细胞生成素的含量。结果显示,按80mg/kg剂量腹腔注射环磷酰胺,每日1次,连用3d,可成功建立骨髓抑制小鼠模型;模型小鼠的白细胞总数、中性粒细胞总数、淋巴细胞总数、血小板总数及血清白介素-6、促红细胞生成素的含量显著降低;红细胞总数、血红蛋白含量及白介素-3的含量降低,但变化不显著;黄芪多糖及各剂量中药复方连续灌胃3d或7d,均可改善模型小鼠的血液生理指标,提高血清白介素-3和促红细胞生成素含量。连续用药3d,以中药复方20g/kg剂量的效果为最佳;连续用药7d,以中药复方10g/kg剂量的效果为最佳。按10g/kg剂量使用中药复方,连续灌胃7d,可极显著提高模型小鼠的白细胞总数、中性粒细胞总数、中性粒细胞百分比、淋巴细胞总数、中间细胞总数、中间细胞百分比、血小板总数以及血清白介素-3、促红细胞生成素的含量(P<0.01);抑制环磷酰胺所致的红细胞总数、红细胞压积、血红蛋白和白介素-6含量降低(P<0.01)。证实由鱼腥草、蒲公英、陈皮、苍术、白芍、白术和当归等组成的中药复方,具有改善环磷酰胺所致骨髓抑制小鼠造血功能的作用,效果优于黄芪多糖;中药复方按20g/kg剂量使用具有作用快速的特点,按10g/kg剂量使用作用稍缓但药效持久。A model of myelosuppressive mice was established by intraperitoneal injection of cyclophos- phamide. The blood ph ＂siological parameters of the model mice, and those treated with Chinese herbal compound（doses of 20 g/kg, 10 g/kg and 5 g/kg） and Astragalus polysaccharide（66 mg/kg） for 3 days or 7 days, were measured by automatic blood cell analyzer. Meanwhile, the content of interleukin-3 （IL-3）, interleukin-6（IL-6） and erythropoietin was measured by ELISA. The results showed that the model of myelo- suppression could be successfully established by daily intraperitoneal injection of 80 mg/kg cyclophosphamide for 3 days. The white blood cell count, neutrophile granulocyte count, lymphocyte count, platelet count and the content of IL-6 and erythropoietin decreased significantly in the myelosuppressive mice. Red blood cell count,and content of hemoglobin and IL-3 decreased slightly. The blood physiological parameters and the content of IL-3 and erythropoietin could be improved to different degrees in the myelosuppressive mice by intragastric administration of Astragalus polysaccharide and each dose of the herbal compound for 3 days or 7 days;the effect of 20 g/kg dose of herbal compound was the best after medicated for 3 days;while the 10 g/kg dose was the best for 7 days. After the myelosuppressive mice receiving daily intragastric administration of 10 g/kg herbal compound for 7 days, the white blood cell count, neutrophile granulocyte count, neutrophile granulocyte percentage, lymphocyte count, intermediate cell count, intermediate cell percentage,platelet count, and the content of IL-3 and erythropoietin could be improved significantly （P〈 0.01） ,and the reduction of red blood cell count,hematocrit,and content of hemoglobin and IL-6 induced by cyclophosphamide could be inhibited significantly（P〈0.01）. The results indicated that the Chinese herbal compound composed by Houttuynia, Taraxacum mongolicum, dried tangerine peel, Atractylodes, white peony root,Atractylodes macrocephala ,Angel

关 键 词：中药复方 骨髓抑制 小鼠 血液生理指标 造血生长因子 

分 类 号：S853.74[农业科学—临床兽医学]