土霉素固体脂质纳米粒的制备及其药剂学性质  

Preparation and pharmaceutical characterization of oxytetracycline solid lipid nanoparticles

作　　者：符华林[1] 周凤[1] 张伟[1] 唐文[1] 卢朝成[1] 胡礼贵 胡俊[1] 付强[1] 邓凤英[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]四川金瑞克动物药业有限公司,四川自贡643010

出　　处：《中国兽医科学》2013年第5期545-550,共6页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

摘　　要：为提高土霉素的稳定性,延长其作用时间,提高药物疗效,以单硬脂酸甘油酯为载体材料,采用乳化蒸发-低温固化法制备土霉素固体脂质纳米粒并对其形态、粒径、Zeta电位、包封率、稳定性和体外释药特性等进行研究。结果显示,所制备的土霉素固体脂质纳米粒外观形态圆整,分散指数为0.512,平均粒径为345.8nm,Zeta电位为-34.7mV,平均包封率为51.25%,载药量为102.5mg/g,4℃放置1个月,包封率、载药量无显著变化。药物的体外释药试验表明制备的纳米粒具有显著缓释作用。结果表明,制备的土霉素固体脂质纳米粒粒径分布范围窄,稳定性较好,对土霉素的释放具有明显的缓释效应。To improve the stability, prolong the action time and improve efficacy of oxytetracycline, ox- ytetracycline solid lipid nanoparticles were prepared by emulsification evaporation-solidification at low temperature with glycerin monostearate. The morphology, particle size, Zeta potentials, entrapment efficiency and release behavior in vitro were investigated. In result,the obtained oxytetracycline solid lipid nanoparti- cles were round with a mean diameter of 345.8 nm,dispersion index of 0. 512,and Zeta potential of -34.7 mV. The entrapment efficiency and drug-loading rate were 51.25% and 102. 5 mg/g respectively. There were no obvious changes in entrapment efficiency and loaded drug at 4 ℃ within one month. The release behavior in vitro of oxytetracycline from solid lipid nanoparticles showed an obvious sustained release effect. The results showed that the oxytetracycline solid lipid nanoparticles had a narrow size distribution and good stability,which can sustain the release of oxytetracycline.

关 键 词：土霉素 固体脂质纳米粒 乳化蒸发-低温固化法 体外释药 

分 类 号：S859.53[农业科学—临床兽医学]