快中子辐照对花生种子胚小叶植株再生的影响  

Effects of fast neutron irradiation on plant regeneration in embryonic leaflet culture of peanut(Arachis hypogaea L.)

作　　者：王晶珊[1] 赵明霞[1] 乔利仙[1] 隋炯明[1] 孔福全[2] 王潇[2] 刘录祥[3] 

机构地区：[1]青岛农业大学生命科学学院/山东省高校植物生物技术重点实验室,山东青岛266109 [2]中国原子能科学研究院,北京102413 [3]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/国家农作物航天诱变技术改良中心,北京100081

出　　处：《中国油料作物学报》2013年第2期148-152,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家自然基金(31101178);国家863计划(2012AA101202);农业部农业公益性行业科研专项(201103007);山东省中青年科学家奖励基金(BS2009NY028);山东省青年基金(ZR2011CQ026)

摘　　要：以花生品种花育22号的成熟饱满种子为试材,采用14MeV不同剂量的快中子(0、9.7、14和18Gy)进行辐照处理。处理后的种子经表面杀菌后,取胚小叶作为外植体先后在添加2,4-D和6-苄氨基嘌呤(BAP)的培养基上进行培养,诱导体胚形成和植株再生。结果表明,体胚诱导率和植株再生率因辐照剂量的不同表现出明显的差异,随辐照剂量的增加,外植体形成体胚的频率及再生植株的频率明显降低。推断快中子辐照花育22号的适宜剂量为9.7～14.0Gy。再生小苗经无菌嫁接驯化后移栽田间,得到了成熟种子。Dry seeds of peanut cultivars Huayu 22 were irradiated by 14 MeV fast neutrons with dosages of 0, 9.7, 14.0 and 18.0Gy respectively. Embryonic leaflets were separated from the irradiated seeds and incubated on MSB5 induction media containing 10mg/L 2,4-D. After 4 weeks culture, the subcultures were transferred to MSB5 media supplemented with 4mg/L BAP. The results showed that the frequencies of somatic embryo formation and plantlet regeneration varied with different dosages of fast neutron irradiation. The somatic embryo induction rates and plant regeneration rates declined with the increase of irradiation dosages. The optimum irradiation dose of fast neutron for Huayu22 was between 9.7Gy and 14.0Gy. The regenerated plantlets were sliced, grafted onto peanut seedlings of Huayu 23 and transplanted into field. The plants grew to maturity and produced viable seeds. ）

关 键 词：花生 快中子辐照 离体培养 体胚诱导 植株再生 

分 类 号：S565.203[农业科学—作物学]