补肾调周法对多囊卵巢大鼠性激素和胰岛素的影响  

Effects of Method of Invigorating Kidney and Regulating Menstruation on Sex Hormone and Insulin Levels in Rats with Polycystic Ovary

作　　者：梁静[1] 孙维峰[1] 周建龙[1] 

机构地区：[1]广州军区广州总医院,广东广州510010

出　　处：《中国中医急症》2013年第5期698-700,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81072915);广东省中医药局立项课题(No.2007204)

摘　　要：目的观察补肾调周法对多囊卵巢(PCOS)大鼠生殖内分泌激素、胰岛素表达的影响,探讨其对PCOS模型大鼠可能的作用机制。方法采用胰岛素(INS)联合人绒毛膜促性腺激素(HCG)造模法制备PCOS模型大鼠。50只大鼠随机分为空白对照组、模型对照组、中药高剂量组、中药中剂量组、西药组。运用放免法测定血清性激素水平的变化;光镜观察卵巢、卵泡发育的影响。结果补肾调周法能显著降低PCOS模型大鼠血清T,LH,E2,INS含量,增加卵巢颗粒细胞层数,卵泡膜细胞层有所变薄。结论补肾调周法可改善多囊卵巢综合征高胰岛素血症和高雄激素血症,全面调节体内内分泌环境,从而改善卵巢功能。Objective： To observe the effect of invigorating kidney and regulating menstruation on expression of genital endocrine hormone and insulin in PCOS rats, and investigate therapeutic actions and possible therapeutic mechanism of the method. Methods： The PCOS rat models were prepared by insulin combined with human chorionic gonadotrophin （HCG）, 50 rats were randomly divided into five group： blank group, model control group, high-dose group of Chinese herb, mid-dose group of Chinese herb, Western medicine group. The levels of serum gonadal hormone were detected by ELISA;Ovarial follicular development were observed by light microscope. Resuits： The method of invigorating kidney and regulating menstruation could increase the thickness of granular cell layer and decrease the thickness of theca cell layer,greatly decrease the level of T.LH,E2 and INS content in PCOS model rats. Conclusion： The method of invigorating kidney and regulatingmenstruation may improve the hyperinsulinemia and hyperandrogenism of PCOS. It can improve the enviroment of internal endocrine and improve the ovarian function.

关 键 词：多囊卵巢综合征 补肾调周法 性激素 胰岛素 

分 类 号：R285.5[医药卫生—中药学]