冠心合剂对大鼠心肌梗死心功能、边缘区凋亡及对Fas、FasL蛋白表达的影响  

The effects of Guanxin Mixture on Heart function,Apoptosis and Fas,FasL Expression in Rats with Acute Myocardial Infarction

作　　者：郑平[1] 陈铁龙[2,3] 祝光礼[2,3] 赫小龙[2,3] 

机构地区：[1]浙江省立同德医院,浙江杭州310000 [2]浙江省杭州市中医院 [3]浙江中医药大学附属广兴医院,浙江杭州310000

出　　处：《中国中医急症》2013年第5期719-721,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2008CB054);杭州市卫生科技计划重点项目(2008Z008);杭州市科技局医院重点专科专病项目(20080333Q23)

摘　　要：目的观察不同剂量冠心合剂对大鼠急性心肌梗死边缘区细胞凋亡及探讨可能作用途径。方法取雄性SD大鼠80只,随机分为模型组、冠心合剂高剂量组[28 g/(kg.d)]、中剂量组[14 g/(kg.d)]、低剂量组[7 g/(kg.d)]和对照组。连续灌胃7 d后,结扎冠状动脉左前降支造成急性心肌梗死模型,对照组不结扎冠状动脉左前降支,手术后4.5 h留取心脏标本。检测心功能、梗死边缘区细胞凋亡以及Fas、FasL蛋白表达量。结果与模型组比较,冠心合剂组可明显提高梗死大鼠心功能、减少心肌细胞凋亡,并能减少Fas、FasL的蛋白表达量。其中冠心合剂大剂量组减少心肌细胞凋亡效果最明显。结论冠心合剂能够提高梗死大鼠心功能、保护缺血诱导的心肌细胞凋亡,其作用机制与降低Fas、FasL蛋白表达水平有关。Objective： To investigate the effects of different dosages of Guanxin Mixture on apoptosis in rats with acute myocardial infarction and its possible mechanism. Methods： 80 male SD rats were randomly divided into 5 groups： model group,sham group, high-dose group （28 g/kg. d Guanxin Heji pretreatment）, medium-dose group （14 g/kg. d Guanxin Heji pretreatment）,low-dose group （7 g/kg. d Guanxin Heji pretreatment）. After being ad- ministered corresponding medicine for 7 days,all groups except sham group underwent coronary ligation of left anterior descending branch to establish acute myocardial infarction models, and sham group only were only given thoracotomy without coronary ligation. Four and half hours after acute myocardial infarction,myocardium tissue were collected to detect the apoptotic index with TUNEL and protein expression of Fas,FasL. Results： The heart function was increased undergoing ligation of left anterior descending in Guanxin Mixture group. The apoPtotic index and protein expression of Fas,FasL decreased significantly in all dose Guanxin Heji groups than those in model group.The high-dose group received the best effect than the others. Conclusion： Guanxin Mixture can elevate heart function and inhibit the myocardial apoptosis by decreasing apoptosis through Fas/FasL pathway.

关 键 词：冠心合剂 急性心肌梗死 心功能 凋亡 FAS FASL 

分 类 号：R285.5[医药卫生—中药学]