胃肠安丸抗炎活性研究  

Anti-inflammatory activity of Weichang’an Pill

作　　者：王磊[1,2] 张静泽[1] 刘振[1] 高文远[1] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]天津中新药业集团股份有限公司乐仁堂制药厂,天津300380

出　　处：《中草药》2013年第8期1017-1021,共5页Chinese Traditional and Herbal Drugs

基　　金：天津市科技攻关计划重大科技工程项目(05ZHGCGX01000)

摘　　要：目的研究胃肠安丸抗炎活性,初步分析其活性成分。方法采用HPLC法对胃肠安丸甲醇提取物进行成分分析;采用二甲苯致小鼠耳肿胀、角叉菜胶致大鼠足趾肿胀、冰醋酸致小鼠毛细血管通透性增加3种急性炎症模型以及大鼠棉球肉芽肿慢性炎症模型,研究胃肠安丸甲醇提取物对急慢性炎症的作用。结果胃肠安丸甲醇提取物中有12个主要成分,分别为来源于木香中的木香烃内酯、去氢木香内酯,来源于厚朴中的厚朴酚、和厚朴酚,来源于枳壳中的柚皮苷、橙皮苷、新橙皮苷,来源于大黄中的芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚。胃肠安丸甲醇提取物800 mg/kg时,对小鼠耳肿胀的抑制率达39.46%,对冰醋酸所致小鼠毛细血管通透性增加的抑制率为35.37%;并对角叉菜胶致炎大鼠足趾致炎后1 h显现抗炎活性;对大鼠棉球肉芽肿显示抑制活性。结论胃肠安丸对动物急慢性炎症均具有较好的抗炎作用。Objective To study the active constituents in the methanol extract of Weichang＇an Pill （MEWP） and their anti-inflammatory activity. Methods The HPLC method was used to anylyze the constituents in MEWE The anti-inflammatory effects of MEWP were observed on the acute anti-inflammatory models of xylene-induced ear edema, carrageenan-induced paw edema, and acetic acid-induced vascular permeability; Meanwhile the chronic anti-inflammatory model of granuloma was induced by cotton pellet implantation. Results Twelve active constituents in MEWP were analyzed, including costunolide and dehydro-^- curcumene from Aucklandiae Radix; naringin, hesperedin, and neohesperedin from Aurantii Fructus; magnolol and honokiol from Magnoliae Officinalis Cortex; aloe-emodin, rhein, emodin, chrysophanol, and physcion from Rhei. Radix et Rhizoma. At the dose of 800 mg/kg, the MEWP showed the inhibitory rate of 39.46% on the ear edema and 35.37% on the increasing of capillary permeability induced by acetic acid in mice. It could decrease the paw edema caused by carrageenan after 1 h and inhibit the granuloma induced by cotton pellet implantation. Conclusion The MEWP could antagonize the reaction of inflammation.

关 键 词：胃肠安丸 抗炎 耳肿胀 足趾肿胀 肉芽肿 

分 类 号：R285.5[医药卫生—中药学]