河南益母草野生居群遗传多样性的SCoT分析  

SCoT analysis on genetic diversity among wild populations of Leonurus artemisia in Henan province

作　　者：张维瑞[1] 韩远记[2] 薛愧玲[1] 袁王俊[1] 尚富德[2] 

机构地区：[1]河南大学中药研究所,河南开封475004 [2]河南大学生命科学学院,河南开封475004

出　　处：《中草药》2013年第8期1022-1026,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30971076);河南省高校创新人才基金资助项目(094100510018)

摘　　要：目的研究河南益母草野生居群遗传多样性。方法利用SCoT分子标记对位于河南伏牛、太行、大别和桐柏4大山脉的8个益母草居群66个样品进行遗传多样性分析。结果 10对引物共扩增出240条带,其中多态性条带为238条,多态性百分率(PPB)为99.17%。Nei’s遗传多样性指数(H)平均值为0.264 2,Shannon’s多态性信息指数(I)平均值为0.39,遗传分化系数(Gst)为0.294 6,种群间基因流为1.197。Mantel Test分析表明,河南益母草居群间的亲缘关系与它们之间的地理距离有着明显的相关性(r=0.366 3,P<0.05)。结论河南野生益母草种群具有较高的遗传多样性,其遗传距离与地理距离存在明显的相关性。Objective To investigate the genetic diversity among the wild populations of Leonurus artemisia in Henan province. Methods The genetic diversity of 66 individuals in eight representational populations of L. artemisia from four areas of Funiu, Taihang, Dabie, and Tongbai Mountains was investigated by SCoT molecular marker technique. Results A total of 240 bands were produced by 10 primers, among which 238 bands were polymorphic bands, and the percentage of polymorphic bands （PPB） was 99.17%. The average value of Nei＇s genetic diversity index （/4） was 0.264 2, Shannon＇s information index （/） was 0.39, genetic differentiation coefficient （Gst） was 0.294 6, and the gene flow （Nm） among populations was 1.197. Mantel Test analysis showed that the significant correlation was found between the geographical and genetic distances among the different populations of L. arternisia （r=0.366 3, P 〈 0.05）. Conclusion The wild populations ofL. artemisia in Henan province have higher genetic diversity. There is a significant correlation between geographical and genetic distances.

关 键 词：益母草 遗传多样性 Nei’s遗传多样性指数 遗传分化系数 基因流 

分 类 号：R282.12[医药卫生—中药学]