RP-HPLC法同时测定不同产地连翘中的7种成分  

Determination of seven components in Forsythia suspensa by RP-HPLC

作　　者：付云飞[1] 李清[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院中药质量控制技术国家地方联合工程实验室,辽宁沈阳110016

出　　处：《中草药》2013年第8期1043-1046,共4页Chinese Traditional and Herbal Drugs

基　　金：2012年辽宁省高等学校中药质量控制关键技术创新团队项目(LT2012018)

摘　　要：目的建立同时测定连翘中咖啡酸、连翘酯苷B、连翘酯苷A、芦丁、金丝桃苷、连翘苷、牛蒡子苷元7种化学成分的HPLC方法。方法采用反相高效液相色谱法,以Inertsil ODS-3 C18(250 mm×4.6 mm,5μm)为色谱柱;流动相为乙腈-0.2%磷酸水,梯度洗脱,检测波长为275 nm,体积流量为1.0 mL/min,柱温为30℃。结果在上述色谱条件下,咖啡酸、连翘酯苷B、连翘酯苷A、芦丁、金丝桃苷、连翘苷、牛蒡子苷元获良好分离,分别在18.24～91.20、5.88～29.40、132.60～663.00、8.34～41.70、1.96～9.80、7.60～38.00、11.34～56.70μg/mL内线性关系良好,平均回收率分别为97.7%、96.7%、102.6%、101.3%、93.2%、91.8%、96.7%,RSD分别为2.3%、1.4%、2.4%、2.2%、1.0%、1.0%、1.3%。结论该方法分离度好,简便快速,可用于同时检测连翘中7种成分的量,为连翘的质量评价提供依据。Objective To establish an HPLC method for the simultaneous determination ofcafferic acid, forsythoside A, forsythoside B, rutin, hyperoside, forsythin, and arctigenin in Forsythia suspensa. Methods The analysis was carried out on an Inertsil ODS-3 CIS column （250 mm x 4.6 mm, 5 pan）. The mobile phase was composed of acetonitrile and 0.2% phosphoric acid aqueous with gradient elution. The detection wavelength was set at 275 nm. The flow rate was 1.0 mL/min at column temperature of 30 ~C. Results Cafferic acid, forsythoside A, forsythoside B, rutin, hyperoside, forsythin, and arctigenin were well separated by this method, and showed a good linearity in the ranges of 18.24--91.20, 5.88--29.40, 132.60--663.00, 8.34--41.70, 1.96--9.80, 7.60--38.00, and 11.34--56.70 ixg/mL, respectively. The average recoveries of the seven components were 97.7%, 96.7%, 102.6%, 101.3%, 93.2%, 91.8%, and 96.7% and the RSD values were 2.3%, 1.4%, 2.4%, 2.2%, 1.0%, 1.0%, and 1.3%, respectively. Conclusion The established method is accurate, reliable, and could be used for the simultaneous determination of the seven components in F. suspense, which provides a scientific basis for the quality evaluation ofF. suspense.

关 键 词：高效液相色谱法 连翘 连翘酯苷 咖啡酸 金丝桃苷 连翘苷 牛蒡子苷元 芦丁 

分 类 号：R286.022[医药卫生—中药学]