肉眼血尿对流式尿沉渣仪红细胞检测的干扰及对策  

The Influence of Gross Hematuria on Sysmex UF-500

作　　者：乔雪峰[1] 崔巍[2] 

机构地区：[1]北京煤炭总医院检验科,北京100028 [2]中国医学科学院北京协和医学院北京协和医院检验科,北京100730

出　　处：《中国医药导刊》2013年第4期564-564,566,共2页Chinese Journal of Medicinal Guide

摘　　要：目的:考察肉眼血尿对流式尿沉渣仪sysmex UF-500的红细胞检测是否造成干扰。方法:挑选9份结果正常的健康体检者新鲜尿标本,每份取2管分为A、B 2组分别与低、高2种浓度肉眼血尿间隔放置,用sysmex UF-500以自动进样模式进行检测并记录红细胞结果。然后观察A、B 2组干扰后结果有无假阳性,并对A组干扰前后红细胞结果进行配对t检验。结果:A组干扰后红细胞结果仍在本室正常参考值范围(<30/ul),无假阳性结果的出现,但t=6.70,p=0.0002说明干扰前后差异有统计学意义;B组干扰后红细胞结果有2例超出本室正常参考值范围(>30/ul),即有2例假阳性。结论:肉眼血尿致流式尿沉渣仪红细胞检测结果较真实结果偏高,差异有统计学意义;且随肉眼血尿浓度的增加可致流式尿沉渣红细胞检测假阳性的出现。Objective：To investigate influence of the gross hematuria on red cell determination of subsequent adjacent specimen. Methods：Choose healthy fresh urine specimens of 9 copies, and gross hematuria to be detected alternately by automatic sampling mode on the Sysmex UF-500 flow cytometer urine sediment analyzer. Results：Group A：difference in red blood cells count before and after jamming has statistics significance t=6.70,P=0.0002;Group B：2 false positive. Conclusion：Interference of gross hematuria on adjacent follow-up specimens erythrocyte detection is obvious;with hematuria concentration increased and even can lead to false positive results.

关 键 词：肉眼血尿 携带污染 尿沉渣 

分 类 号：R446.12[医药卫生—诊断学]