EGF与TGF-β1调控MMP20基因表达的研究  

MMP20 Gene Expression Induced by EGF and TGF-β1 in Ameloblasts

作　　者：于会杰[1] 王玉敏[2] 韩婷婷[2] 李东亮[2] 李伯翰[2] 张娟娟[1] 孙岩[1] 高玉光[2,3] 

机构地区：[1]潍坊医学院口腔医学研究所,山东潍坊261053 [2]滨州医学院口腔医学院 [3]滨州医学院附属医院口腔科

出　　处：《潍坊医学院学报》2013年第2期107-109,161,共3页Acta Academiae Medicinae Weifang

基　　金：国家自然科学基金(30973327);山东省自然科学基金(ZR2010HM076))

摘　　要：目的观察表皮生长因子(EGF)与TGF-β1对小鼠成釉细胞金属基质蛋白酶-20(MMP20)基因表达的调控作用,并探讨其分子作用机制。方法利用Real time-PCR法分析EGF,TGF-β1对成釉细胞MMP20基因表达的影响;阻断MAPK通路,利用Real time-PCR法分析EGF,TGF-β1对MMP20基因表达的影响;利用双荧光素酶基因报告系统分析EGF,TGF-β1对成釉细胞MMP20基因启动子转录活性的影响。结果与对照组相比,EGF与TGF-β1均显著上调MMP20基因表达;加入MAPK抑制剂后,显著抑制了EGF与TGF-β1对MMP20基因表达的作用。双荧光素酶报告基因检测系统显示:与对照组相比,EGF与TGF-β1显著促进MMP20基因启动子的转录活性。结论 EGF通过P38通路与ERK通路上调MMP20的表达;TGF-β1通过p38通路与JNK通路上调MMP20的表达。Objective To investigate the regnlatory effects of EGFandTGF-β1 induced matrix metalloproteinase20（MMI）20） ex- pression in ameloblasts. Methods RT-PCR was used to observe the influence of EGF and TGF-β1 on MMP20 gene expression in amelo- blasts. Blocking MAPK path, RT-PCR was used to determinate the influence of EGF andTGF-β1 on MMP20 gene expression; Dual Iuciferase analysis was used to observe the effects of EGFandTGF-β1on the transcriptional activity of MMP20 promoter. Results Compared with the control group,MMP20 expression was risen by EGF and TGF-β1. After joining MAPK inhibitor, MMP20 gene expression was significantly in- hibited. By the Dual-I.uciferase Reporter Assay System, compared with the control group, the transcriptional activity of MMP20 which was stimulated by EGF and TGF-β1 had risen. Conclusion EGF enhanced MMP20 expression mediated by P38 and ERK signaling pathway in ameloblasts,and TGF-β1 enhanced MMP20 expression mediated by P38 and JNK signaling pathway in ameloblast.

关 键 词：EGF TGF-Β1 基质金属蛋白酶-20 P38 MAPK 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]