慢性乙型肝炎患者外周血CD4^+CD25^+调节性T细胞的研究  

The Study on CD4^+ CD25^+ Regulatory T Cells in the Peripheral Blood of Chronic Hepatitis B Patients

作　　者：王丽丽[1] 李群[1] 窦雯雯[2] 张杰[1] 李增彩[1] 

机构地区：[1]潍坊医学院传染病学教研室,山东潍坊261053 [2]潍坊医学院附属医院感染性疾病科

出　　处：《潍坊医学院学报》2013年第2期152-154,共3页Acta Academiae Medicinae Weifang

摘　　要：目的测定慢性乙型肝炎(CHB)患者外周血CD4^+CD25^+调节性T细胞(Treg)的水平并探讨其临床意义。方法流式细胞术检测40例慢性乙型病毒性肝炎患者(根据B超将其分为2组,其中B超提示慢性肝病改变的CHB慢性肝病组20例,提示肝硬化改变的CHB肝硬化组20例),20例健康对照组(Nc)对照外周血中CD4^+CD25^+,Treg的变化。结果外周血中CD4^+CD25^+Treg占CD4淋巴细胞的比例在CHB慢性肝病组为(12.00±1.47)%,CHB肝硬化组为(11.14±1.07)%,与NC组(9.65±1.01)%相比差异有显著性(P<0.05),CHB慢性肝病组与CHB肝硬化组间比较差异无显著性(P>0.05)。结论 Treg与CHB患者体内存在持续HBV感染关系密切,而是否参与乙肝后肝硬化的发病机制需要深入研究。Objective To determinate CD4 ＋ CD25＋ regulatory T cells（Treg） levels of patients with chronic hepatitis B（CHB） in peripheral blood and to explore its clinical significance. Methods Flow cytometry detected 40 pa- tients with chronic hepatitis B patients （ Be divided into two groups according to B-mode ultrasound, 20 patients with chronic liver disease changes in B-mode ultrasound,20 patients with liver cirrhosis in B-mode ultrasound were assayed）. Twenty healthy subjects were used as control. Results The proportion of peripheral blood CD4 ＋ CD25＋Treg accounted CD4 lymphocytes in CHB chronic liver disease group presented （ 12.00 ± 1.47 ） % , while the liver cirrhosis patients presented（ 11.14 ± 1.07 ） % ,which were both higher than those（9.65 ± 1.01 ） % of the controls（ P 〈0.05 ） ,there was no significant difference between chronic hepatitis B and chronic hepatitis B cirrhosis group （ P 〉 O. 05 ）. Conclusion These results indicate that the CD4 ＋ CD25 ＋ Treg plays a role in the persistent HBV infection,and whether to participate in the hepatitis B cirrhosis pathogenesis requires in-depth study.

关 键 词：CD4+CD25+调节性T细胞 乙型病毒性肝炎 慢性 肝硬化 

分 类 号：R512.62[医药卫生—内科学]