决明子愈伤组织培养  

Callus Induction from Leaves and Stems of Semen Cassiae

作　　者：黄凤兰[1] 曾健寒[1] 布和巴特[2] 张更 邢春[1] 赵永[1] 卜志刚[1] 李跃[1] 郭雅馨[1] 陈亚平[1] 

机构地区：[1]内蒙古民族大学生命科学学院,内蒙古通辽028000 [2]内蒙古民族大学,蒙医药学院内蒙古通辽028000 [3]鄂尔多斯市农牧业科学研究院,内蒙古东胜017000

出　　处：《内蒙古农业科技》2013年第2期25-26,29,共3页Inner Mongolia Agricultural Science and Technology

基　　金：内蒙古民族大学教育教学研究项目(No.2009053)

摘　　要：决明子是一种重要的蒙药,目前国内还没有有关植物生长调节剂(PGR)对决明子培养影响的研究报道。实验的目的是以决明子的茎段和叶片为外植体,诱导愈伤组织,为决明子的快繁和细胞培养奠定基础。实验结果如下:将种子用75%酒精处理45S、0.1%升汞处理5min,然后接种到30g/L蔗糖+7g/L琼脂的培养基中以获得无菌苗。无菌苗叶片诱导愈伤组织阶段最适培养基为MS+蔗糖30g/L+琼脂7g/L+6-BA 2mg/L+NAA 0.6mg/L;茎段诱导愈伤组织阶段最适培养基为MS+蔗糖30g/L+琼脂7g/L+6-BA3mg/L+NAA0.7mg/L。Semen Cassiae is an important medicinal plant in Mongolian medical practice.In this paper, influence of plant growth regulator （PGR） on callus induction from stems and leaves were determined by experiments. The optimized protocol was established as follows.The seeds of Semen Cassiae were disinfected with 75% alcohol for 45 S,and then 0.1% HgCl2 for 5 min; afterwards,they are placed onto the medium containing 30 g/L sucrose and 7 g/L agar.When we obtained the aseptic seedlings, the leaves were removed for callus induction and transferred onto medium containing of MS,30g/L sucrose,7g/L agar,2 mg/L 6- BA and 0.6 mg/L NAA;simultaneously,the stems were removed and transferred onto the medium containing MS, 30g/L sucrose, 7g/L agar,3mg/L 6-BA and 0.7 mg/L NAA.Our work will promote multiple propagation and cell culture of Semen Cassiae,since there has been no report about tissue culture of this medicinal plant in China

关 键 词：决明子 愈伤组织 植物生长调节剂 

分 类 号：S567[农业科学—中草药栽培]