1株蛇足石杉根际铁载体细菌的分离、筛选与鉴定  

Isolation,Screening and Identification of Sidero-Carrier Bacteria from Huperzia serrata Rhizosphere

作　　者：肖艳红[1] 李菁[1] 刘祝祥[1] 杜次[1] 李贵[1] 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000

出　　处：《微生物学杂志》2013年第2期63-67,共5页Journal of Microbiology

基　　金：国家自然科学基金(31260081);植物资源保护与利用湖南省高校重点实验室开放基金(JSK200904);吉首大学校级科研项目资助(Jdy12038)

摘　　要：对蛇足石杉根际铁载体细菌进行了分离、筛选与鉴定,以期筛选到潜在高效促生细菌。采用梯度稀释涂平板法从蛇足石杉根际土中分离细菌,再通过CAS检测平板分析,从中筛选出1株产生铁载体能力较强的菌,并结合16S rDNA基因系统发育分析对可产铁载体的蛇足石杉根际细菌初步鉴定。从蛇足石杉根际中筛选到1株产铁载体细菌JSX 389,经鉴定为Lysinibacillus属菌株。In order to obtain potential highly effective plant growth-promoting bacteria, sidero-carrier bacteria （SCB） were isolated, screened and identified from rhizosphere of Huperzia serrata using gradient dilution method, among them a strong SCB strain was rescreened by using the chrome azurol sulfonate （CAS） agar plate assay. The strain was initially identified combined with phylogenetic analysis according to 16S rDNA. A siderophores-producing strain JSX389 was isolated from H. serrata rhizosphere and identified as a strain of the genus of Lysinibacillus.

关 键 词：蛇足石杉 细菌 铁载体 16S RDNA 

分 类 号：Q93-31[生物学—微生物学]