高Vir毒力根癌农杆菌AGL-1菌株介导丝状真菌转化研究进展和应用  

Progress&Application of High Vir-Toxicity Agrobacterium tumefaciens Strain AGL-1 Filamentous Fungi Mediated Transformation

作　　者：陈璨[1] 蒋冬花[1] 齐育平[1] 曹丽凌[1] 黄娜[1] 孙青[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《微生物学杂志》2013年第2期80-85,共6页Journal of Microbiology

基　　金：国家自然科学基金(31070008;31270061)

摘　　要：根癌农杆菌介导的真菌转化(ATMT),是分离真菌基因和分析基因功能的重要方法。目前有多种农杆菌菌株可运用于ATMT法,例如LB4404、C58C1、EHA104、AGL-1等。其中AGL-1菌株因其具有Vir毒力高、转化效率高、稳定性好等诸多优点,近年来有关AGL-1菌株高效转化丝状真菌的应用越来越多。就其转化机制、转化过程、转化率影响因素的最新研究进展进行概述。Agrobacterium tumefaciens-mediated transformation （ATMT） is an important method to isolate fungal gene and to analyze gene function. Many strains of A. tumefaciens are ATMT applicable, such as strains LB4404, C58C1, EHA104, and AGL-1 etc. Among them AGL-1 has been more and more utilized in ATMT in recent years because of the characteristics of high Vir-toxicity, high transformation efficiency and good stability. Advances in transformation mechanism, process, and influencing factors were summarized in this paper.

关 键 词：根癌农杆菌 AGL-1菌株 丝状真菌 T-DNA 影响因素 

分 类 号：Q939.96[生物学—微生物学]