GC/MS法对南方红豆杉种皮抑制物分析研究  

Study on Seed Coat Dormancy Substances of Taxus Mairei (Lemee et Levl.) S.Y.Hu ex Liu by GC/MS

作　　者：张春椿[1] 年慧慧[1] 李效贤[1] 沈晨薇[1] 应明花[1] 王亚芸[1] 熊耀康[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《浙江中医药大学学报》2013年第4期446-450,共5页Journal of Zhejiang Chinese Medical University

基　　金：浙江省科技厅项目(2012C23109);浙江省中医药重点研究项目(2011ZZ003);浙江省自然基金项目(LQ12H28003);浙江省教育厅科研项目(Y20110650)~~

摘　　要：[目的]研究南方红豆杉种皮内源抑制物的种类。[方法]采用系统溶剂分离法对南方红豆杉种皮提取液进行分离,并对乙酸乙酯相、甲醇相、石油醚相进行甲酯化后采用气相色谱-质谱联用仪进行鉴定分析。[结果]南方红豆杉种皮石油醚相中含有8种化合物,乙酸乙酯相中含有6种化合物,甲醇相中含有5种化合物,其主要内源抑制物为十六烷酸、十八烷酸、9-十八碳烯酸、9,12-十八碳二烯酸等,以(Z)9-十八碳烯酸含量最高,在乙酸乙酯相中存在1种未知醇类。[结论]该研究为南方红豆杉种子的综合利用提供科学依据,同时也为完善南方红豆杉种子休眠机理提供理论依据。[Objective] To study the constituents of endogenesis inhibitory substances in seed embryo of Taxus mairei（Lemee et Levi.） S. Y.Hu ex Liu. [Methods] The extracts of seed embryo were separated into four components using four menstruums by systematic solvent separation method, then each component was analyzed by GC-MS.[Results] Five compounds were separated and identified in petroleum ether component, nine compounds were i- dentified in ethyl acetate component, six compounds were identified in methanol component. The main endogenesis inhibitory substances were Hexadecanoate acid, Octadecanoic acid, 9-Octadecenoic acid, 9,12-Octadecadienoic acid.Among which,（Z）-9-Octadecenoic acid was presented the highest relative content. The ethyl acetate and methanol component contained a kind of unknown material,respectively. [Conclusion] The study could provide the scientific basis for comprehensive utilization, and provide the theory basis for perfecting dormancy mechanism of Taxus mairei（Lemee et Levl.）S. Y. Hu ex Liu.

关 键 词：GC-MS 南方红豆杉 内源抑制物 

分 类 号：R282.71[医药卫生—中药学]