一株高效纤维素降解菌的筛选及其产酶条件优化  

Screening of Microbes with High Quality of Cellulose-Decomposing Enzyme and Optimization of the Conditions for Cellulase Production

作　　者：冯海玮[1,2] 周培[1,2,3] 毛亮[1,2] 时唯伟[1,3] 支月娥[1,2] 

机构地区：[1]上海交通大学农业与生物学院 [2]上海交通大学环境科学与工程学院 [3]农业部都市南方(重点)实验室,上海200240

出　　处：《上海交通大学学报（农业科学版）》2013年第2期24-29,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海崇明科技攻关专项(10DZ1960103);农业部公益性行业(农业)科研专项(200903056);国家科技支撑崇明设施栽培生境低碳关键技术集成与示范(10DZ1960102)

摘　　要：为获得高效纤维素降解菌株,以CMC-Na为唯一碳源进行筛选,从土壤及腐烂秸秆中分离到26株纤维素降解菌。分别测定其滤纸崩解、CMC-刚果红水解圈、CMCase(羧甲基纤维素酶)和FPase(滤纸片酶)活性等指标,从中筛选出产纤维素酶能力最强的JSD-1放线菌。通过测定不同条件下JSD-1放线菌产纤维素酶的活性,得到其最佳产酶条件。结果表明,当温度为35℃、发酵液初始pH为6.5、接种量为8%及转速为180r/min时,CMCase和FPase分别在发酵第5天和第4天有最大的产酶活性,为59.19和31.68U/mL。In order to obtain microbes with high quality of cellulose-decomposing enzyme, twenty six strains of cellulose-decomposing microbes were isolated from soil and rotten straw by using the media supplemented with CMC-Na as the sole carbon resource. For these strains,the following experiments were conducted incIuding the decomposition of filter-paper, decoloration of Congo red, production of CMCase and FPase. The actinomycete JSD-1 was selected due to its strongest ability to degrade cellulose and conditions for cellulase production of JSD-1 are also established. The results showed that the optimum cultural temperature for JSD-1 was 35℃ ,the pH for initial fermentation is 6. 5, the inoculation volume is 8%, the rotate speed is 180 r/min and the suitable culture time is 5 and 4 d for the production of CMCase and FPase, respectively. Under these conditions,the activity of CMCase and FPase reached at 59.19 and 31.68 U/mL, respectively.

关 键 词：纤维素降解 菌株 筛选 放线菌 产酶活性 

分 类 号：K703[历史地理—历史学]