围着床期小鼠胚胎表达肿瘤易感基因101(TSG101)  

Expression of tumor susceptibility gene 101(TSG101)in murine embryos during peri-implantation period

作　　者：谢琪璇[1] 黄卉卉[1,2] 蒋启荣 高桥祐司 秋山泰身[5] 秦俊文[1,2] 

机构地区：[1]暨南大学再生医学教育部重点实验室,广东广州510632 [2]暨南大学生命科学技术学院发育与再生生物学系,广东广州510632 [3]广东省动物防疫监督总所,广东广州510230 [4]国立成育医疗研究中心生殖·细胞医疗研究部,东京157-8535 [5]东京大学医科学研究所分子发癌分野,东京108-8639

出　　处：《暨南大学学报（自然科学与医学版）》2013年第2期134-138,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(81270752);广东省自然科学基金项目(9451063201002016);教育部留学回国人员科研启动基金项目(23610022);中央高校基本科研业务费专项资金项目(21610406;21611432);暨南大学引进人才科研启动基金项目(51209004)

摘　　要：揭示肿瘤易感基因101(TSG101)在围着床期胚胎中的时空表达型。方法:收集囊胚、培养获得着床前、着床后以及长开的小鼠胚胎。利用全胚原位杂交以及全胚免疫染色方法检测围着床期小鼠胚胎中TSG101的时空表达型。结果:TSG101表达于围着床期胚胎的內细胞块和滋养层细胞中,其中内细胞块细胞中的表达更强。结论:围着床期胚胎表达TSG101,暗示TSG101在胚胎着床过程中发挥作用。Aim ：To detect the temporal and spatial expression pattern of tumor susceptibility gene 101 （ TSG101 ） in embryos during peri-implantation. Methods： Blastocysts, pre-, post-implantation and out- growthing murine embryos were collected. Whole-mount in situ hybridization and whole-mount immunohistochemical analyses were performed to determine the expression pattern of TSG101 during peri-implanration. Results： During the course of development from early blastoeyst stage to outgrowing embryo stage, TSGI01 was present in both inner cell mass （ICM） and trophectoderm. However, stronger signal of TSG101 was observed in ICM. Conclusion：TSG101 was present in murine embryos during peri-implantation period.： The temporal and spatial expression of TSG101 in murine embryos during the peri-implantation period suggested that TSGI01 might play a pivotal role in the processes of embryonic implantation.

关 键 词：TSG101 表达型 胚胎 围着床期 

分 类 号：Q492.6[生物学—生理学]