4NQO诱导大鼠腭黏膜癌模型建立的实验研究  

A rat model of palatal mucosa carcinoma induced by 4-nitroquinolinel-oxide water solution

作　　者：岳阳丽[1] 季旭东[1] 刘进忠[1] 

机构地区：[1]郑州大学口腔医学院口腔病理科,河南郑州450052

出　　处：《暨南大学学报（自然科学与医学版）》2013年第2期139-143,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：河南省教育厅自然科学研究计划项目(2011A320058)

摘　　要：目的:建立与人腭黏膜癌及癌前病变发生相似的动物模型。方法:体积分数为0.5%的四硝基喹啉-1-氧化物(4-nitroquinoline 1-oxide,4NQO)水溶液涂抹Wistar大鼠腭部黏膜至28周,肉眼及组织学观察癌变全过程。结果:随4NQO作用时间的延长,小鼠腭黏膜相继出现白色斑块、红白相间斑块、乳头状新生物和浸润性肿块等改变,组织学上经历了单纯性上皮增生、异常增生、原位癌、浸润性癌的病理过程,用药16、20、24、28周时腭癌的发生率分别为0、11.1%、44.4%、100%,未见远处转移。结论:4NQO涂抹法诱发大鼠腭癌模型建立方法简单、靶器官代表性强、病变典型,癌变时间稳定集中,且与人类口腔癌病变过程相似,是研究口腔癌的理想动物模型。Aim：To establish a rat model for palatal mucosa carcinoma and precancerous lesions that is histologically and pathologically comparable to the human counterpart. Methods： 0.5% 4NQO solu- tion was applied to the rat＇s palate mucosa once two days, and the animals were observed until the 28th week. The rats were killed and their palatal mucosas were biopsied for pathological analyses. Results： Gross changes included leukoplakia, erythroplakia appearances, papillary hyperplasia and invasive carcinoma of the palatal mucosa of experimental group rats during carcinogenesis. The palate lesions had undergone a classical progress of squamous cell carcinoma including simple epithelial hyperplasia, dysplasi- a,in situ carcinoma and invasive cancer. The incidence of palate cancer in rats treated with 4NQO for 16 weeks, 20 weeks, 24 weeks, and 28 weeks was 0, 11.1%, 44. 4% and 100%, respectively. No metastases were found. Conclusion ：4NQO can reliably induce rat palatal preneoplastic lesions, carcinoma the rat palatal mucosa. The process of carcinogenesis and the biological behavior parallel to those of hu- man oral carcinoma. The target organ is typical, and the method is handy. This model is simple, convenstable and repeatable as an ideal animal model of oral cancer.

关 键 词：4-硝基喹啉-1-氧化物 腭黏膜癌 大鼠 动物模型 

分 类 号：R379.8[医药卫生—病原生物学]