微孔板结合化学发光法快速测定覆盆子中总糖蛋白的抗氧化活性  

Rapid Determination of Antioxidant Activities of Glycoprotein of Rubus chingii Hu.by Chemiluminescence Method Coupled with Microwell Plate

作　　者：雷利芳[1] 游静[2] 曾华金[2] 杨冉[1] 屈凌波[1,3] 

机构地区：[1]郑州大学化学与分子工程学院,河南郑州450001 [2]郑州大学药学院,河南郑州450001 [3]河南工业大学化学化工学院,河南郑州450001

出　　处：《发光学报》2013年第5期650-655,共6页Chinese Journal of Luminescence

摘　　要：在碱性条件下,覆盆子总糖蛋白对鲁米诺-过氢化氢、鲁米诺-邻苯三酚-氢氧化钠和鲁米诺-硫酸铜-过氧化氢化学发光体系具有明显的抑制作用。基于这一特点,本文将微孔板与化学发光法结合,在优化条件下,建立了覆盆子总糖蛋白清除H2O2、O2-和.OH自由基能力的快速检测方法,结果表明该方法具有样品用量少、操作简单、检测速度快等优点,适用于药物中有效成分的活性检测及筛选。Based on the phenomenon that glycoprotein can obviously inhibit the chemical luminescence （CL） signal of luminol-H202 system, luminol-pyrogallol-sodium hydroxide system and luminol-H2O2-copper sulfate system in basic medium, a simple and rapid chemiluminescence method int egrated with microwell plate was developed for evaluating of the scavenge effects of glycoprotein of Rubus chingii Hu. on hydrogen peroxide （H2O2 ）, superoxide anion （O2-） and hydroxyl radical （ @ 0H）. The experiment data indicated the proposed method described in this paper has proved to be fairly simple, reproducible and rapid and would be a valuable tool for fast screening of antioxidant activity of herbal.

关 键 词：微孔板 化学发光法 覆盆子 糖蛋白 抗氧化 

分 类 号：R917[医药卫生—药物分析学]