纳米金-碳管敏感膜对溶血素的快速电信号检测  

Fast Electrical Signal Detection for Listeriolysin by Sensitive Membrane of Nanogold-modified Carbon Nanotubes

作　　者：贺胜男[1] 樊彦恩[1] 武帅[1] 杨良保[2] 刘洪林[2] 

机构地区：[1]中国电子科技集团公司第三十八研究所,安徽合肥230088 [2]中国科学院合肥智能机械研究所纳米功能材料与传感器件研究中心,安徽合肥230031

出　　处：《宿州学院学报》2013年第2期67-72,共6页Journal of Suzhou University

基　　金：国家重点基础研究发展计划(973)"应用纳米技术去除饮用水中微污染物的基础研究"(2011CB933700);国家自然科学基金"高灵敏选择磁性核壳纳米SERS探针的构筑及其对农药残留的检测研究"(21271136)

摘　　要：在O2等离子体处理和空气气氛中500℃高温热处理方法的辅助下,简便制备了单壁碳管-纳米金(SWNT-Au)复合网络敏感膜。等离子体处理的SWNTs高密度且均一地组装了正电荷Au纳米颗粒。热处理过程不仅去除了SWNTs表面吸附的所有杂质,使其与叉指金电极形成良好的接触;而且导致Au颗粒表面熔化并聚集,残余的SWNTs管束变细。这些变化极大地改变了敏感膜作为换能器的电流-电压行为,放大了其对抗体-抗原相互作用事件的敏感性。利用敏感膜作为换能元件,构建了基于SWNT-Au网络的电信号生物免疫传感器,实现了对李斯特氏菌溶血素的高敏感、快速检测,检测限能达到约2ng.mL-1左右。We proposed a new strategy to facilely hybridize the SWNTs and Au NPs. The SWNT-Au network was used to bridge the interdigital electrode for constructing an immunosensor. Positively-charged Au NPs can be assembled high-density on the oxidized SWNTs. And we carried out the annealing at 500℃ in air for the Au NPs surface melting and aggregation. The impurities adsorbed on the SWNT-Au was removed and a good contact was formed between the SWNT-Au and the electrode. The voltage and current transducer behavior of this sensitive film was greatly changed and further enlarged its sensitivity to antibody-antigen interaction events. As energy conversion devices using sensitive membrane, the electrical signals of biological immune sensor was monitored based on SWNT-Au neural network. A highly sensitive,rapid detection of Listeria hemolysin,was realized and the detection limit can reach about 2 ng · mL.

关 键 词：纳米金 单壁碳纳米管 电信号 免疫传感器 李斯特氏菌溶血素 

分 类 号：TB383.1[一般工业技术—材料科学与工程]