血卟啉单甲醚联合紫杉醇对宫颈癌Hela细胞杀伤效应的研究  

The effects of hematoporphyrin monomethyl ether connected with paclitaxel on human cervical carcinoma cell lines

作　　者：张宾美 罗荣城[2] 李黎波[2] 张兰英[2] 陈晓华 蔡思娜[4] 

机构地区：[1]广东省中山板芙医院妇产科,广东中山528400 [2]南方医科大学南方医院肿瘤中心,广东广州510515 [3]广东省广州番禺区中心医院肿瘤科,广东广州511400 [4]南方医科大学第三附属医院肿瘤科,广东广州510630

出　　处：《中国临床医生杂志》2013年第5期37-39,共3页Chinese Journal For Clinicians

基　　金：广东省自然科学博士启动基金(NO:S2011040000786)

摘　　要：目的初步探讨血卟啉单甲醚(HMME)介导的光动力疗法(HMME-PDT)联合紫杉醇(PTX)对人宫颈癌Hela细胞的杀伤效应。方法将宫颈癌细胞株Hela分为6组,a:空白对照组,b:化疗组,c:饱和光动力组,d1:化疗+低剂量光敏剂组,d2:化疗+中剂量光敏剂组,d3:化疗+高剂量光敏剂组。MTT法检测细胞生长抑制率。结果 d3组与a、b、c组及d1、d2组相比,差异均有显著性(P<0.01);d1、d2、d3各组之间相比,差异有显著性(P<0.05);b组及c组与a组相比,差异有显著性(P<0.05)。结论 HMME-PDT联合紫杉醇对体外培养的人宫颈癌Hela细胞有明显的杀伤效应,有可能作为一种新的治疗手段应用于临床。Objective To investigate the effects of Photodynamic Therapy （PDT） combining paclitaxel（PTX） on pro-liferation in cervical cancer. Method Hela cells were divided into six groups： a group：Control group, b group： PTX,e group： HMME-PDT, dl group： Low HMME dose PDT＋PTX,d2 group：Middle HMME dose PDT＋PTX,d3 group：High HMME dose PDT＋PTX. The survival rates of the cells were measured by MTF assay. Result There were statistically significant difference between d3 group with other groups （ P〈0.01 ）. Also there was statistically significant difference among the dl ,d2 ,d3 groups （P〈0.05）. Finally there was statistically significant difference after the b group or the e group compared with the a group （P〈0.05）. Conclusion The killing effects on human cervical carcinoma cell lines are obvious by HMME-PDT connected with paclitaxel, which may become a new thera-py method to be using in clinical.

关 键 词：宫颈癌 血卟啉单甲醚 光动力疗法 紫杉醇 

分 类 号：R711.74[医药卫生—妇产科学]