人参皂苷Rh_2的C-20构型确证  

Determination of C-20 steric configuration for ginsenoside Rh_2

作　　者：徐光[1] 阮征 白景 姜永涛[1] 

机构地区：[1]烟台大学药学院药物化学研究室,山东烟台264005 [2]山东绿叶制药有限公司化学中试研究室,山东烟台264003

出　　处：《中国现代医生》2013年第14期50-52,55,共4页China Modern Doctor

摘　　要：目的确证人参次苷H主成分人参皂苷Rh2的结构与空间构型。方法单晶X-射线衍射,HPLC、13C-NMR比较与其C-20的R型异构体的差异。结果 HPLC保留时间显示人参皂苷H中主成分人参皂苷Rh2与酸降解R型异构体不同,二者是两种不同成分;13C-NMR数据与文献报道的20(S)-人参皂苷Rh2一致。单晶X-射线衍射结构分析也证实其为20(S)人参皂苷Rh2。结论人参次苷H主成分为20(S)-人参皂苷Rh2。Objective To confirm the structure and steric configuration of the main component of secondary-ginsenoside-H, 20（S）-ginsenoside Rh2. Methods Through the single crystal X-ray diffraction analysis, HPLC, and 13C-NMR data analysis compared with R-type epimer after acid degradation treatment. Results The retention time of HPLC show the main component of secondary-ginsenoside-H, ginsenoside-Rh2, and the ginsenoside Rh2 from PQS （Panax quinquefolium saponins from stems and leaves） after acid degradation treatment to be two different epimers. 13C-NMR spectroscopic data showed the ginsenoside Rh2 separated from secondary-ginsenoside-H has the same data with reported in literatures. And the single crystal X-ray diffraction analysis of chemical structure also prove to be 20 （S）-ginsenoside Rh2. Conclusion The C-20 of ginsenoside Rh2 epimer, a main component of secondary-ginsenoside H, is S-type.

关 键 词：20(S)-人参皂苷Rh2 差向异构体 单晶X-射线衍射 

分 类 号：R285[医药卫生—中药学]