血管紧张素Ⅱ对骨髓间充质干细胞定向诱导为角质形成细胞调控作用的实验研究  

An experimental study on the differentiation of bone marrow mesenchymal stem cells into keratinocytes and its role of ANGⅡ in the process

作　　者：徐媛[1] 刘宏伟[1] 程飚[1] 肖丽玲[1] 肖静[1] 吴帆[1] 李升红[1] 

机构地区：[1]暨南大学附属第一医院整形外科.再生医学教育部重点实验室,广东广州510630

出　　处：《中国美容医学》2013年第7期739-742,共4页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(30772257;30973127;81272100);中央高校基本科研业务费专项资金资助项目(编号:21611016);广州市科技计划项目(编号:2012J4100044)

摘　　要：目的:探讨骨髓间充质干细胞(MSCs)分化为角质形成细胞的可能性及在此过程中血管紧张素Ⅱ(AngⅡ)对其的调控作用。方法:抽取Wistar大鼠的骨髓,经全骨髓法分离、纯化MSCs,鉴定后建立MSCs细胞模型,用成胶质细胞诱导培养基诱导其为角质形成细胞,显微镜下观察其形态学变化。以添加了AngⅡ的成角质形成细胞诱导培养基组与单纯成角质形成细胞诱导培养基组在诱导MSCs 7天、10天后行角蛋白10(CKP10)免疫组织化学染色及流式细胞仪检测,并进行对比观察分析。结果:培养的MSCs具有成脂、成骨分化的能力。MSCs可以成功诱导为角质形成细胞,添加AngⅡ的诱导组与对照诱导组CKP10免疫组化染色均有阳性表达,但添加AngⅡ组阳性细胞数高于对照组。流式细胞仪检测显示CKP10阳性细胞百分率,AngⅡ组为80.62%,对照组(32.46%),两者相差显著(P<0.05)。结论:MSCs可以诱导分化为角质形成细胞,ANGⅡ对MSC的成角质形成细胞分化有显著的促进作用,这一作用可能是AngⅡ促进创面愈合的机制之一。Objective To investigate the feasibility of differentiation of bone marrow mesenchymal stem cells （MSCs） into epithelial cells and the role of ANGII in the process. Methods MSCs were harvested from porcine marrow, and then they were isolated and purified. Cultured MSCs were analyzed by flow cytometry. At the same time, in vitro differentiation into osteogenic and adipogenic phenotypes was performed. Then MSCs were divided into two groups to be induced into epithelial cells with epithelium induction medium while the other added ANGII. After 7 and 10 days culture, MSCs were stained with cytokeratin 10 Abs. Results We successfully got the MSCs and observe the phenomenon that MSCs could be induced into epithelial cells. More cells in the group of MSCs added with ANGII turned into epithelial cells then the other group. Conclusion MSCs were closely correlated with the regeneration of epithelial cells and wound healing. ANGII promoted wound healing since it enhanced the rate of MSCs＂ turning into epithelial cells.

关 键 词：骨髓间充质干细胞 角质形成细胞 创面愈合 

分 类 号：Q813.1[生物学—生物工程]